pPR3-C yeast hybrid plasmid

Cat: MOFY-0123-FY27
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Species SourceYeast
Size2 µg
CompositionPromoter: ADH1 promoter;
Replicon: pUC ori, F1 ori, 2μ ori;
Terminator: CYC1 terminator;
Plasmid classification: yeast cell plasmids; yeast hybrid plasmids; two-hybrid plasmids;
Plasmid size: 6620bp;
Plasmid tags: C-HA, C-NubG;
Prokaryotic resistance: Amp;
Screening marker: TRP1;
Cloned strain: DH5a;
Culture conditions: 37 degrees;
Expression host: yeast;
Culture conditions: 30°C, YPD, aerobic;
Remarks: High copy plasmid.
BufferRefer to COA

Plasmid Information

Product OverviewpPR3-C is a plasmid used for membrane protein yeast two-hybrid experiments. The DUALmembrane system is designed to detect and characterize interactions involving integral membrane proteins and membrane-associated proteins in yeast. The DUALmembrane Pairwise Interaction Kit enables you to: Study interactions between membrane proteins (integrated or membrane-associated) and membrane proteins or soluble proteins Map domains or amino acids critical for the interaction Use membrane proteins as screening cDNA libraries Bait for finding new interacting proteins (requires purchase of a separate cDNA library from Dualsystems).
Regulatory StatusFor Research Use Only
ShippingDry ice
StorageStore at -20 °C.
References1. Nakamura, Y., Ishii, J., & Kondo, A. (2013). Rapid, facile detection of heterodimer partners for target human G-protein-coupled receptors using a modified split-ubiquitin membrane yeast two-hybrid system. PLoS One, 8(6), e66793.
2. Nakamura, Y., Takemoto, N., Ishii, J., & Kondo, A. (2014). Simultaneous method for analyzing dimerization and signaling of G-protein-coupled receptor in yeast by dual-color reporter system. Biotechnology and Bioengineering, 111(3), 586-596.
For Research Use Only | Not For Clinical Use.
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