SHuffle T7 E. coli competent cells (MOFY-0822-FY513)
Cat: MOFY-0822-FY513
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Specifications
| Description | The SHuffle T7 E. coli strain is a derivative of K12. A copy of the disulfide bond isomerase DsbC gene is integrated into the chromosome of this strain, which can promote the correct folding of disulfide bond-containing proteins; in addition, DsbC is also a molecular chaperone, which can help the correct folding of disulfide bond-free proteins. At the same time, the strain can reduce the background expression of the target gene and is suitable for the prokaryotic expression of the virulence gene. |
| Gene Types | F´ lac, pro, lacIq / Δ (ara-leu)7697 araD13fhuA2 lacZ::T7 gene1 Δ (phoA)Pvull phoR ahpC* galE (or U) galKλatt::pNEB3-r1-cDsbC (SpecR, laclq) ΔtrxBrpsL150 (StrR)Δgor Δ (malF)3 |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 1×108 cfu/μg DNA |
| Note | 1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. 3. During induction, IPTG concentration can be selected (0.1-10 mM can be used). 4. In order to obtain the required amount of protein, the induction time, temperature and IPTG concentration need to be optimized by the experimenter. 5. The SHuffle T7 E. coli strain is resistant to streptomycin and spectinomycin, and cannot be used for the transformation of plasmids with resistance to streptomycin and spectinomycin. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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