XL10-Gold chemically competent cells (MOFY-0822-FY467)
Cat: MOFY-0822-FY467
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Specifications
| Description | XL10-Gold chemical competitive cells XL10-Gold is currently the highest transformation efficiency of competitive cells, developed by Stratagene specifically for the transformation of large plasmids or precious ligation products or super-competent cells for library construction. The XL10-Gold strain is a Hte (high transformation efficiency) genotype. Hte is a host genotype developed by Stratagene to specifically improve the competent transformation efficiency and the ability to transform large plasmids. It has been successfully used in the construction of 40kd plasmids. |
| Gene Types | TetR Δ (mcrA)183 Δ (mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F´ proAB lacIqZΔM15 Tn10 (TetR) Amy CamR] |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 2×109 cfu/μg DNA |
| Note | 1. Competent cells should be thawed slowly in ice, and the target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. Be gentle when mixing plasmids or ligated products. 2. Be gentle when mixing plasmids or ligation products. 3. Transforming high concentrations of plasmids or high-efficiency ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating. 4. The XL10-Gold strain is resistant to <40 µg/ml chloramphenicol, but sensitive to 100 µg/ml chloramphenicol. 5. XL10-Goldcompetent cells use conventional transformation methods, and the transformation efficiency can reach 2×109 cfu/μg DNA; if there are higher requirements, you can try the standard protocol recommended by Stratagene. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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