Mouse Anti-ABT1 Antibody (CBMOAB-00193HCB)
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Lot Number
- Product List
- Specifications
- Application Information
- Target
- Relate Reference Data
| Sub Cat | Clonality | Species Reactivity | Application | Clone | Conjugate | Size | |
| CBMOAB-00193HCB | Monoclonal | C. elegans (Caenorhabditis elegans), Cattle (Bos taurus), Chimpanzee (Pan troglodytes), Ferret (Mustela Putorius Furo), Frog (Xenopus laevis), Marmoset, O. mykiss (Oncorhynchus mykiss), Rhesus (Macaca mulatta), Zebrafish (Danio rerio) | WB, ELISA | MO00193HB | 100 µg | ||
| CBMOAB-34900FYA | Monoclonal | Rhesus (Macaca mulatta) | WB, ELISA | MO34900FYA | 100 µg | ||
| CBMOAB-64534FYA | Monoclonal | Zebrafish (Danio rerio) | WB, ELISA | MO64534FYA | 100 µg | ||
| MO-AB-15567W | Monoclonal | Chimpanzee (Pan troglodytes) | WB, ELISA | MO15567W | 100 µg | ||
| MO-AB-34323W | Monoclonal | Ferret (Mustela Putorius Furo) | WB, ELISA | MO34323W | 100 µg | ||
| MO-AB-50275W | Monoclonal | Marmoset | WB, ELISA | MO50275W | 100 µg | ||
| MO-AB-06823R | Monoclonal | Cattle (Bos taurus) | WB, ELISA | MO06823R | 100 µg | ||
| MO-AB-01153H | Monoclonal | Frog (Xenopus laevis) | WB, ELISA | MO01153C | 100 µg | ||
| MO-AB-10596Y | Monoclonal | O. mykiss (Oncorhynchus mykiss) | WB, ELISA | MO10596Y | 100 µg |
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | C. elegans (Caenorhabditis elegans), Cattle (Bos taurus), Chimpanzee (Pan troglodytes), Ferret (Mustela Putorius Furo), Frog (Xenopus laevis), Marmoset, O. mykiss (Oncorhynchus mykiss), Rhesus (Macaca mulatta), Zebrafish (Danio rerio) |
| Clone | MO00193HB |
| Specificity | This antibody binds to C. elegans ABT1. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Product Overview | Mouse Anti-C. elegans ABT1 (clone MO00193HB) Antibody (CBMOAB-00193HCB) is a mouse antibody against ABT1. It can be used for ABT1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Protein ABT-1, isoform a; abt-1 |
| Gene ID | 182849 |
| UniProt ID | S6EZS3 |
| Protein Refseq | The length of the protein is 1558 amino acids long. The sequence is show below: MSSVLSSFSSARRSIRNIWQKDLNYFRSNHPFFILIFIVLSLAFYNVWWHNSPTRDQSGDASKFHLFDNFRLSKQKFVKMIVCIPETVVDYRFSTVIDAIHLTHLDYPHLEFTKKLEKAKNKLEDGEVDFLVLFKTLIETGKKIEYKIISEQGVFDKHCIQCDVHRKKTWGNAVEAQVVVNRFLYWLVKSRGFNKRSPHWQLSNVYVKHSGNDPMFLKRQSDLFLLWILLLPICSRLIDLWNNDRSSGYYNMFLSLHARRFHYFIAKFVFWYGIALIISLIVFIVNVLVVPAVFCSTYLQFVVCYAACIISFAIFLATTFPGSPLFAKIVGFLSMVAGEFGFGTVYHGIGFFQLCVRHFGKSEFDGPWIDAVCTLCMTGNCVLLITVSIYFDTYFCLFTMEPLAFYFPLEKDYWMPEARSPQLDTFFVKQIILLKPGRSEITVKKAHDEILPAKTSTGSKYIPPTPIVKGVPLVVLYGICKRIENHWRVKQVSLTVHLGEVVTLYGHHGCGSGEILSIIAGQMKPEYGEVMMEQSQRPLMISKTTDVPNVNYLNVIQYLRLISKMRGVSASTSHIDEMLEELDLIKVKNRSLDLLSTTQKERLRIAAVFVGQPDLVLIDFPTMECLPEWKFMVFRFIEKRKEKRSIIVSSYDPEETEAISDKVVLMSEGYVVLNGSCEAFKHSINSVFEIRIWPNNRFTDEQIKGMMKTLTLGDNQMKNDARFFETPNGKIRVTLPILYRRSIPLILRELDAVHEKFRIEFYELGKPNIHDIYANACYEHQQLDPLSTFDQLRDHYTKQQPLNKKTYFFKNMIHIFKDGRFLIETAAVVGLFLILTVIILLSYHSVESDSHQTREISLTSYESPITIYCDECEKEVIEGVTFEKKSRVLQLENNQAVLYWKDTAKADKELITVSRGDALFTMIVQNFAVSQMISKITGKHPQIKLFLESENVRTATEISGFSTVFNDISSSKAAMILTESYSLMYITAVFQMFVYTFSVVLPLRLVSTNMGPQSMILPWPRYVYFGFIYVFQLVVFLVIAIILGFAVLSMGFFATNTTSCYLRFLSAWFLSYASTLPLIYFLVFNIQNTKSVIPIILAISSLSVTFPNLVASFSAENQVSLQSLIQFVSWSCMLNPPSSLQVLAAFLNAGESLEKNSGTITSLILFCGAQFWIIVIFVLVIFQPGFSYIQQYWFKSSTVLYSSFERGTTLKPGARIKNSYIFVTNPLGTMEDAQDKGASGNIPNRPAAGTEPVQKPTSDYIAGSSMSIDDMSYITNYEENLVEKMATMTSWNDKQEVPVDNSATFLMGDQTKLKSALLTTIAKINKNEHIAFVPIVENISTIYTPFELLENAAYCNGFEITDDHISYLLDAFDLKEHANTQIKLLPCIDRRILMLLAKLVIKPNIVIIDQLEMFLPHSKMMTVWSFCARLRKNGLGIIYTSHHSGFAEHTATCCAHMYKGQFVRNLLPTSIKAKCTVLEVVPKTASDPKVLLHMLQKEMEESVKLPSTSRNITLAMYKDGVETIEKILELLRMLSPQIKRYSLRSGNCDEFLASAFGK. |
Relate Reference Data
IF
Figure 1 Subcellular localization of mABT1. COS7 cells were transfected with pEGFP-mABT1; after 24 h, cells expressing EGFP proteins were monitored by fluorescence microscopy under phase contrast (A) and dark field (B).
WB
Figure 2 mABT1 interacts with TBP. Coimmunoprecipitation of mABT1 and TBP. COS7 cells were transfected with pcDNA3-myc-mABT1, and lysate was prepared. Immunoprecipitations with control rabbit IgG (lane 1) or anti-TBP antibody (lane 2) were performed, and the immunocomplex was analyzed with antibodies against TBP (α-TBP blot) and c-Myc (α-Myc blot). TBP and Myc-mABT1 in the whole cell lysate are shown in lane 3.
WB
Figure 3 mABT1 stimulates transcription in transfected cells. Coexpression of mABT1 stimulates luciferase activity from the reporter plasmid pTATA-Luc. pTATA-Luc (0.1 μg) was cotransfected in COS7 cells with different amounts of pcDNA3-myc-mABT1 (0 to 2 μg). The total amount of plasmids used for each transfection was normalized with pcDNA3-myc.
IF
Figure 4 Subnuclear localization of ABT1 and ABTAP. A: Subnuclear localization of ABT1. pDsRed-ABT1 was transfected in COS7 cells. Arrows indicate nucleoli.