AibGenesis™ Mouse Anti-APE1 Antibody (CBMOAB-00250CR)


Cat: CBMOAB-00250CR

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Lot Number

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Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-00250CR Monoclonal Yeast, A. thaliana (Arabidopsis thaliana), C. elegans (Caenorhabditis elegans), Malaria parasite WB, ELISA MO00250CR 100 µg
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CBMOAB-00563HCB Monoclonal C. elegans (Caenorhabditis elegans) WB, ELISA MO00563HB 100 µg
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CBMOAB-2262FYC Monoclonal A. thaliana (Arabidopsis thaliana) WB, ELISA MO2262FC 100 µg
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MO-AB-11011H Monoclonal Malaria parasite WB, ELISA MO11011C 100 µg
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MO-DKB-02195W Polyclonal A. thaliana (Arabidopsis thaliana) WB 100 µg
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityYeast, A. thaliana (Arabidopsis thaliana), C. elegans (Caenorhabditis elegans), Malaria parasite
CloneMO00250CR
SpecificityThis antibody binds to Yeast APE1.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationVacuole; Other locations

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionResident vacuolar enzyme that catalyzes the removal of amino acids from the N-terminus of peptides and proteins. Also acts as the major cargo protein of the cytoplasm-to-vacuole targeting (Cvt) pathway. The precursor form of aminopeptidase 1 (prApe1) assembles into dodecamers and the propeptide mediates the aggregation of dodecamers into higher multimers. The multimers are then recognized via the propeptide by their receptor ATG19, and ATG19 further interacts with ATG11, which tethers the APE1-ATG19 complex to the pre-autophagosomal structure (PAS). The cargo-receptor complex (also Cvt complex) is selectively enwrapped by a double-membrane structure termed the Cvt vesicle under vegetative growth conditions and by a similar but larger double-membrane structure termed the autophagosome under nitrogen starvation conditions. The Cvt vesicle or the autophagosome fuses with the vacuolar membrane and release its content in the vacuolar lumen. In the vacuole, prApe1 is processed into mature aminopeptidase 1 (mApe1). (From uniprot, under CC BY 4.0)
Product OverviewMouse Anti-Yeast APE1 Antibody is a mouse antibody against APE1. It can be used for APE1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesVacuolar aminopeptidase 1; EC 3.4.11.22; Aminopeptidase yscI; Leucine aminopeptidase IV; LAPIV; Lysosomal aminopeptidase III; Polypeptidase; Vacuolar aminopeptidase I; APE1; API LAP4 YSC1; YKL103C
UniProt IDP14904
Protein RefseqThe length of the protein is 514 amino acids long. The sequence is show below: MEEQREILEQLKKTLQMLTVEPSKNNQIANEEKEKKENENSWCILEHNYEDIAQEFIDFIYKNPTTYHVVSFFAELLDKHNFKYLSEKSNWQDSIGEDGGKFYTIRNGTNLSAFILGKNWRAEKGVGVIGSHVDALTVKLKPVSFKDTAEGYGRIAVAPYGGTLNELWLDRDLGIGGRLLYKKKGTNEIKSALVDSTPLPVCRIPSLAPHFGKPAEGPFDKEDQTIPVIGFPTPDEEGNEPPTDDEKKSPLFGKHCIHLLRYVAKLAGVEVSELIQMDLDLFDVQKGTIGGIGKHFLFAPRLDDRLCSFAAMIALICYAKDVNTEESDLFSTVTLYDNEEIGSLTRQGAKGGLLESVVERSSSAFTKKPVDLHTVWANSIILSADVNHLYNPNFPEVYLKNHFPVPNVGITLSLDPNGHMATDVVGTALVEELARRNGDKVQYFQIKNNSRSGGTIGPSLASQTGARTIDLGIAQLSMHSIRAATGSKDVGLGVKFFNGFFKHWRSVYDEFGEL.
For Research Use Only | Not For Clinical Use.
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