Cat: CBMOAB-0111FYC
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- Product Details
- Relate Reference Data
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | A. thaliana (Arabidopsis thaliana) |
| Clone | MO0111FC |
| Specificity | This antibody binds to Arabidopsis AT4G23470 / AT4G23480. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Several rounds of confirmation of Y2H positivity resulted in a total of three novel proteins interacting with ELF3: the PLAC8 domain-containing protein AT4G23470, LUL4, and AtGLDP1. AT4G23470 was recovered in two independent clones and LUL4 was recovered in three independent clones. The PLAC8 domain protein AT4G23470 is encoded by a gene within the QTL interval on chromosome 4, but the affinity of this protein did not vary among the various ELF3-polyQs. |
| Product Overview | Mouse Anti-Arabidopsis AT4G23470 / AT4G23480 (clone MO0111FC) Antibody (CBMOAB-0111FYC) is a mouse antibody against AT4G23470 / AT4G23480. It can be used for AT4G23470 / AT4G23480 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | PLAC8 family protein; Putative uncharacterized protein At4g23480; At4g23470 At4g23480 |
| UniProt ID | Q67XV0 |
| Protein Refseq | The length of the protein is 199 amino acids long. The sequence is show below: MSRYVCCAGYMPCSGRCGEAKCPQLCLATEVFCCFANSVASTRFLLQDEFQIQTTKCDNCIIGFMVCLSQVACIFSIVACIVGMDELSEASQILTCCSDMVYCTVCACMQTQHKMEMDKRDGKFGPQPMAVPPAQQMSRFDQATPPAVGYPPQQGYPPSGYPQHPPQGYPPSGYPQNPPPSAYSQYPPGAYPPPPAYPK. |
Reference
| Reference | 1. Press, M. O., & Queitsch, C. (2017). Variability in a short tandem repeat mediates complex epistatic interactions in Arabidopsis thaliana. Genetics, 205(1), 455-464. 2. Maximilian Oliver Press, & Queitsch, C. (2016). The variable ELF3 polyglutamine tract mediates complex epistatic interactions in Arabidopsis thaliana. bioRxiv, 061564. 3. Cabreira-Cagliari, C., Dias, N. D. C., Bohn, B., Fagundes, D. G. D. S., Margis-Pinheiro, M., Bodanese-Zanettini, M. H., & Cagliari, A. (2018). Revising the PLAC8 gene family: from a central role in differentiation, proliferation, and apoptosis in mammals to a multifunctional role in plants. Genome, 61(12), 857-865. |
Figure 1 Y2H screen identifies new interactors of ELF3. Yeast carrying indicated vectors were spotted in fivefold dilutions onto C −Leu −Trp (C−LT) or C −Leu −Trp −His −Ade (C−LTHA) media. For each protein X, experiments were repeated with at least two independent PJ69-4α + pGADT7-X transformants with similar results.
For Research Use Only | Not For Clinical Use.