Mouse Anti-C. elegans GCN1 Antibody (CBMOAB-04295HCB)


Cat: CBMOAB-04295HCB
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Size:
Conjugate:
 Inquiry
  • Product Details

Specifications

Host speciesMouse (Mus musculus)
Species ReactivityC. elegans (Caenorhabditis elegans)
CloneMO04295HB
SpecificityThis antibody binds to C. elegans GCN1.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationCytosol

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionActs as a positive activator of the GCN2 protein kinase activity in response to amino acid starvation (PubMed:8497269, PubMed:24333428). Component of the GCN1-GCN20 complex that forms a complex with GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of GCN2, and hence stimulating GCN2 kinase activity (PubMed:7621831, PubMed:9234705, PubMed:15722345). Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator GCN4, by promoting the GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2) on ''Ser-52'', and hence allowing GCN4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:8497269, PubMed:10801780, PubMed:11350982, PubMed:15722345).
Product OverviewMouse Anti-C. elegans GCN1 Antibody is a mouse antibody against GCN1. It can be used for GCN1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesProtein GCN-1; gcn-1
UniProt IDB3CJ34
Protein RefseqThe length of the protein is 2651 amino acids long. The sequence is show below: MSDDEIKSEHVQEKTENLSEEDALKEEIRRFSSAVSNPSLRAHGMAYAALTKLIERRSTEIPAQFVKGLVKMSIANSIRLYGQPKSFQAVSKLLQVLAKRDPISTLKSLLQTITTALPIIPNLTESHSLSQVPTMKWLLACLDGNSCAENAVEIFGALGAIAYWMSASDKAWRVFTKKLAQLFAKNPEISNVFTAKIGEFSKENPEKSLALISAFGALGAAKTPEIAPIFVEIFTKSQLLPKLRPPQFFVTRISKFAVFLQKSPDFETNLLPNMKKALLRSPEVAIFGIRGLLEHISFSLDFCAQDLLKSTISSLCNTDPEIREAGIGIVVALSRKSEISVIQKILNTLLDQFSAAKASEIKCSLMEGVGAIAKVVEAQDIDKIAEDVISKTAKADKESHDGTIEAQWNAAAEWAIRLSKPAISLTNAFKEASKLAPAVKYIGYRVLADILERRSMTSLPEGIDVKPLLTELETGPKGELCSIGLALLILRTTKKGSIEHTKAWSRASHAEALLKFLQEKPLSQTKWQEAIAWAKLVELVILDRPVVNKTAPPSYSLPSIKPLSLLLFWPNWKVRQQASKSLLRILSTENALFAEALADLIFSETTNGTIDQLLRRVPAEISMKNPQISGISGCPTPVDSWQVPGEWYVSTLRLLLTPKEPELDKLAIHTLLLASVQRLVEVDGSVWLRWVHEHRESKTWQASEVFRETAIQNVLQCKDRGVRNTALVTLIALHDERLRADLWKHVDASIKQLEVTDYGRITEQEISIYKCAEGQLYNTRVLDFDEAAGIKGTKGTSIQDQMAEIQLIRELAAKRQKEGKLSAKQKQVMEKELAAEKDTRDALRELYVTAEAKLDECRAMVAADAAGAFARPEILFEYCMPLTRSLLVSEEAARLFLAYRDACFEHSDDYLEELVGSNWLRVIGAHHRIPGWNDEFLDSALKRMFSLLNERAFVIDSGDDDDDEPVIFDDEIVGANHLTLLYPMIRIILKNSQRFSDNSRQDALQLLQSAIHKKFLRDRDVLNLPMEHYASMLFEHFSLDSGATNAFKATQQLFRLANDTNDIGDRVIGMVREILAYVGNEQPSVRGAALEILGAPHLLMRIVVELGIEQSISKEILVRIHVARHDPIEAVSEIAERLWIENHLQVKQVIGAMLVDECVSPSPLVRQSAAHAMVTFIEEHPNEMPAILTKFDETYKDLVLIREPIYDDVGRLQREAIDESDRRSGIGQTLVLLAGLCRQEEAEQLIRIVAPDGLSDRAQECRNELRNAAVETIRRHGAACMLRLLPLLEKLSDETPAQDDNRRQGLVVLLGTLAQYIDSTEKVKGIVARLVEALGTPSQTVQESVSRCLAPLVPKIRQDAKDLVSKLQWTLFEAETYGERRGAAYGIAGLMKGMGIIALKDTDLLGSIHKNMEDKKSPKHREGGLLALEILCCTIGKLFEPYILKALPSLLITFGDTDSNVRQSAEDTARAMMASMTVYGTKLVLPVLIVAIDDDSWRTKCAATELLGSMAFCAPRQLSACLPNIVPKLIEILADSSSKVQKSGEKALQQIARVVRNPEILGVTNQLMAGLLDPANKTSAALQAVLNTKFIHYIDAPSLALMMPIVRRAFEDRLSETRRVAAQIISNIYSLTENKDMEPYLAHMVPGLQRSLLDPVPEIRAVSARALGAVVSKSGGSTSENLRAQVIPWLKEKLVSPQSTVDRSGAAQGLCEVLAGAGTEQLEYVMPEIIHATESTDVSAETRDGYILMYIYLPMTFGDRFVPYLPQVVPPILKALADENEYVRASALKAGQRLISQYCSHARKLLLPQLQLALMDENWRIRYASVQLIGDFLFNISGITGKSTSSTADEDDTMGMEQAGKVIVRALGQKDRDRVLAGLYLTRSDVALVVRQAAGHVWKMVVSNTPRTLREVTKILFEMVVDSLASTCDERQQMGARCLGELVRKMGDKVINDILPVLDANQKSEEVAKRVGVAIALHEIIGNMSKEVTNHYLGAIVAPVRRAICDESELVREAAADTFTVLYHVVGNEALDEIICPLLEQLTPEQDHILAGLCDVMRQNSRSMLPYLLPKLTKPPVNVHALCSLASVSGDSLSRQLPKVLDALLAACETNDESDPMIESCEKVVIAVTDEDGIPVLVDYLIQKASQDENVPAAVLLSTFIAKSGVSLAEMAEEVLPGLLNLYTSPNPQIVDHAIAAAVALTQSMDQRELLSVLPVVKKAINIIVAGAKGQQIPGFTHPKSLQPLVVMLRESILQGQIEMKALAAECLGMVVKVSDVAALKAHVVNITGPLIRVLGDRFPANVKLPIIETLSKLLDKVDAMLRPFLPQLQSTFLKALQEPTSRPVRLAAGGALARLLKLHPKPEATMTELLKLLATSTDQQLIESSLATARALIATCGQKMSPTTIDEIYRVTELIYSENVENPTELDASLTACSGALLGETIAQKSDWKTAQNCVLSGIESPSTSARGRQAKACALQQLCSSNGDELWASEANSACRSAFTSAFTSPDPIVASAALRGASHVLKVSIDRDLMSAVARSLNHASTDVRKTAGIALGHVGHSADLPNDILKLIVPQLINGCKESNSQVRAASELALVHALKMTQNEDRFEAYRNTLEGVVQRNLDETRVSLARVVRAGDVDLEPLDTILNVY.
See other products for " GCN1 "
MO-AB-03727WMouse Anti-Rhesus GCN1 Antibody (MO-AB-03727W)
CBMOAB-01452CRMouse Anti-Yeast GCN1 Antibody (CBMOAB-01452CR)
MO-AB-10540WMouse Anti-Chimpanzee GCN1 Antibody (MO-AB-10540W)
For Research Use Only | Not For Clinical Use.
Online Inquiry