Mouse Anti-GCN1 Antibody (CBMOAB-04295HCB)
Cat: CBMOAB-04295HCB
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
- Product List
- Specifications
- Application Information
- Target
Sub Cat | Clonality | Species Reactivity | Application | Clone | Conjugate | Size | |
CBMOAB-04295HCB | Monoclonal | C. elegans (Caenorhabditis elegans), Chimpanzee (Pan troglodytes), Rhesus (Macaca mulatta), Yeast | WB, ELISA | MO04295HB | 100 µg | ||
CBMOAB-01452CR | Monoclonal | Yeast | WB, ELISA | MO01452CR | 100 µg | ||
MO-AB-03727W | Monoclonal | Rhesus (Macaca mulatta) | WB, ELISA | MO03727W | 100 µg | ||
MO-AB-10540W | Monoclonal | Chimpanzee (Pan troglodytes) | WB, ELISA | MO10540W | 100 µg |
Specifications
Host species | Mouse (Mus musculus) |
Species Reactivity | C. elegans (Caenorhabditis elegans), Chimpanzee (Pan troglodytes), Rhesus (Macaca mulatta), Yeast |
Clone | MO04295HB |
Specificity | This antibody binds to C. elegans GCN1. |
Format | Liquid or Lyophilized |
Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
Purity | > 90% was determined by SDS-PAGE |
Purification | Purified with Protein A or G affinity chromatography |
Cellular Localization | Cytosol |
Application Information
Application | WB, ELISA |
Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
Introduction | Acts as a positive activator of the GCN2 protein kinase activity in response to amino acid starvation (PubMed:8497269, PubMed:24333428). Component of the GCN1-GCN20 complex that forms a complex with GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of GCN2, and hence stimulating GCN2 kinase activity (PubMed:7621831, PubMed:9234705, PubMed:15722345). Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator GCN4, by promoting the GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2) on ''Ser-52'', and hence allowing GCN4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:8497269, PubMed:10801780, PubMed:11350982, PubMed:15722345). |
Product Overview | Mouse Anti-C. elegans GCN1 Antibody is a mouse antibody against GCN1. It can be used for GCN1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
Alternative Names | Protein GCN-1; gcn-1 |
UniProt ID | B3CJ34 |
Protein Refseq | The length of the protein is 2651 amino acids long. The sequence is show below: MSDDEIKSEHVQEKTENLSEEDALKEEIRRFSSAVSNPSLRAHGMAYAALTKLIERRSTEIPAQFVKGLVKMSIANSIRLYGQPKSFQAVSKLLQVLAKRDPISTLKSLLQTITTALPIIPNLTESHSLSQVPTMKWLLACLDGNSCAENAVEIFGALGAIAYWMSASDKAWRVFTKKLAQLFAKNPEISNVFTAKIGEFSKENPEKSLALISAFGALGAAKTPEIAPIFVEIFTKSQLLPKLRPPQFFVTRISKFAVFLQKSPDFETNLLPNMKKALLRSPEVAIFGIRGLLEHISFSLDFCAQDLLKSTISSLCNTDPEIREAGIGIVVALSRKSEISVIQKILNTLLDQFSAAKASEIKCSLMEGVGAIAKVVEAQDIDKIAEDVISKTAKADKESHDGTIEAQWNAAAEWAIRLSKPAISLTNAFKEASKLAPAVKYIGYRVLADILERRSMTSLPEGIDVKPLLTELETGPKGELCSIGLALLILRTTKKGSIEHTKAWSRASHAEALLKFLQEKPLSQTKWQEAIAWAKLVELVILDRPVVNKTAPPSYSLPSIKPLSLLLFWPNWKVRQQASKSLLRILSTENALFAEALADLIFSETTNGTIDQLLRRVPAEISMKNPQISGISGCPTPVDSWQVPGEWYVSTLRLLLTPKEPELDKLAIHTLLLASVQRLVEVDGSVWLRWVHEHRESKTWQASEVFRETAIQNVLQCKDRGVRNTALVTLIALHDERLRADLWKHVDASIKQLEVTDYGRITEQEISIYKCAEGQLYNTRVLDFDEAAGIKGTKGTSIQDQMAEIQLIRELAAKRQKEGKLSAKQKQVMEKELAAEKDTRDALRELYVTAEAKLDECRAMVAADAAGAFARPEILFEYCMPLTRSLLVSEEAARLFLAYRDACFEHSDDYLEELVGSNWLRVIGAHHRIPGWNDEFLDSALKRMFSLLNERAFVIDSGDDDDDEPVIFDDEIVGANHLTLLYPMIRIILKNSQRFSDNSRQDALQLLQSAIHKKFLRDRDVLNLPMEHYASMLFEHFSLDSGATNAFKATQQLFRLANDTNDIGDRVIGMVREILAYVGNEQPSVRGAALEILGAPHLLMRIVVELGIEQSISKEILVRIHVARHDPIEAVSEIAERLWIENHLQVKQVIGAMLVDECVSPSPLVRQSAAHAMVTFIEEHPNEMPAILTKFDETYKDLVLIREPIYDDVGRLQREAIDESDRRSGIGQTLVLLAGLCRQEEAEQLIRIVAPDGLSDRAQECRNELRNAAVETIRRHGAACMLRLLPLLEKLSDETPAQDDNRRQGLVVLLGTLAQYIDSTEKVKGIVARLVEALGTPSQTVQESVSRCLAPLVPKIRQDAKDLVSKLQWTLFEAETYGERRGAAYGIAGLMKGMGIIALKDTDLLGSIHKNMEDKKSPKHREGGLLALEILCCTIGKLFEPYILKALPSLLITFGDTDSNVRQSAEDTARAMMASMTVYGTKLVLPVLIVAIDDDSWRTKCAATELLGSMAFCAPRQLSACLPNIVPKLIEILADSSSKVQKSGEKALQQIARVVRNPEILGVTNQLMAGLLDPANKTSAALQAVLNTKFIHYIDAPSLALMMPIVRRAFEDRLSETRRVAAQIISNIYSLTENKDMEPYLAHMVPGLQRSLLDPVPEIRAVSARALGAVVSKSGGSTSENLRAQVIPWLKEKLVSPQSTVDRSGAAQGLCEVLAGAGTEQLEYVMPEIIHATESTDVSAETRDGYILMYIYLPMTFGDRFVPYLPQVVPPILKALADENEYVRASALKAGQRLISQYCSHARKLLLPQLQLALMDENWRIRYASVQLIGDFLFNISGITGKSTSSTADEDDTMGMEQAGKVIVRALGQKDRDRVLAGLYLTRSDVALVVRQAAGHVWKMVVSNTPRTLREVTKILFEMVVDSLASTCDERQQMGARCLGELVRKMGDKVINDILPVLDANQKSEEVAKRVGVAIALHEIIGNMSKEVTNHYLGAIVAPVRRAICDESELVREAAADTFTVLYHVVGNEALDEIICPLLEQLTPEQDHILAGLCDVMRQNSRSMLPYLLPKLTKPPVNVHALCSLASVSGDSLSRQLPKVLDALLAACETNDESDPMIESCEKVVIAVTDEDGIPVLVDYLIQKASQDENVPAAVLLSTFIAKSGVSLAEMAEEVLPGLLNLYTSPNPQIVDHAIAAAVALTQSMDQRELLSVLPVVKKAINIIVAGAKGQQIPGFTHPKSLQPLVVMLRESILQGQIEMKALAAECLGMVVKVSDVAALKAHVVNITGPLIRVLGDRFPANVKLPIIETLSKLLDKVDAMLRPFLPQLQSTFLKALQEPTSRPVRLAAGGALARLLKLHPKPEATMTELLKLLATSTDQQLIESSLATARALIATCGQKMSPTTIDEIYRVTELIYSENVENPTELDASLTACSGALLGETIAQKSDWKTAQNCVLSGIESPSTSARGRQAKACALQQLCSSNGDELWASEANSACRSAFTSAFTSPDPIVASAALRGASHVLKVSIDRDLMSAVARSLNHASTDVRKTAGIALGHVGHSADLPNDILKLIVPQLINGCKESNSQVRAASELALVHALKMTQNEDRFEAYRNTLEGVVQRNLDETRVSLARVVRAGDVDLEPLDTILNVY. |
For Research Use Only | Not For Clinical Use.
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