Mouse Anti-GCN1 Antibody (CBMOAB-04295HCB)


Cat: CBMOAB-04295HCB
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Lot Number

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Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-04295HCB Monoclonal C. elegans (Caenorhabditis elegans), Chimpanzee (Pan troglodytes), Rhesus (Macaca mulatta), Yeast WB, ELISA MO04295HB 100 µg
CBMOAB-01452CR Monoclonal Yeast WB, ELISA MO01452CR 100 µg
MO-AB-03727W Monoclonal Rhesus (Macaca mulatta) WB, ELISA MO03727W 100 µg
MO-AB-10540W Monoclonal Chimpanzee (Pan troglodytes) WB, ELISA MO10540W 100 µg

Specifications

Host speciesMouse (Mus musculus)
Species ReactivityC. elegans (Caenorhabditis elegans), Chimpanzee (Pan troglodytes), Rhesus (Macaca mulatta), Yeast
CloneMO04295HB
SpecificityThis antibody binds to C. elegans GCN1.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationCytosol

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionActs as a positive activator of the GCN2 protein kinase activity in response to amino acid starvation (PubMed:8497269, PubMed:24333428). Component of the GCN1-GCN20 complex that forms a complex with GCN2 on translating ribosomes; during this process, GCN1 seems to act as a chaperone to facilitate delivery of uncharged tRNAs that enter the A site of ribosomes to the tRNA-binding domain of GCN2, and hence stimulating GCN2 kinase activity (PubMed:7621831, PubMed:9234705, PubMed:15722345). Participates in the repression of global protein synthesis and in gene-specific mRNA translation activation, such as the transcriptional activator GCN4, by promoting the GCN2-mediated phosphorylation of eukaryotic translation initiation factor 2 (eIF-2-alpha/SUI2) on ''Ser-52'', and hence allowing GCN4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (PubMed:8497269, PubMed:10801780, PubMed:11350982, PubMed:15722345).
Product OverviewMouse Anti-C. elegans GCN1 Antibody is a mouse antibody against GCN1. It can be used for GCN1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesProtein GCN-1; gcn-1
UniProt IDB3CJ34
Protein RefseqThe length of the protein is 2651 amino acids long. The sequence is show below: MSDDEIKSEHVQEKTENLSEEDALKEEIRRFSSAVSNPSLRAHGMAYAALTKLIERRSTEIPAQFVKGLVKMSIANSIRLYGQPKSFQAVSKLLQVLAKRDPISTLKSLLQTITTALPIIPNLTESHSLSQVPTMKWLLACLDGNSCAENAVEIFGALGAIAYWMSASDKAWRVFTKKLAQLFAKNPEISNVFTAKIGEFSKENPEKSLALISAFGALGAAKTPEIAPIFVEIFTKSQLLPKLRPPQFFVTRISKFAVFLQKSPDFETNLLPNMKKALLRSPEVAIFGIRGLLEHISFSLDFCAQDLLKSTISSLCNTDPEIREAGIGIVVALSRKSEISVIQKILNTLLDQFSAAKASEIKCSLMEGVGAIAKVVEAQDIDKIAEDVISKTAKADKESHDGTIEAQWNAAAEWAIRLSKPAISLTNAFKEASKLAPAVKYIGYRVLADILERRSMTSLPEGIDVKPLLTELETGPKGELCSIGLALLILRTTKKGSIEHTKAWSRASHAEALLKFLQEKPLSQTKWQEAIAWAKLVELVILDRPVVNKTAPPSYSLPSIKPLSLLLFWPNWKVRQQASKSLLRILSTENALFAEALADLIFSETTNGTIDQLLRRVPAEISMKNPQISGISGCPTPVDSWQVPGEWYVSTLRLLLTPKEPELDKLAIHTLLLASVQRLVEVDGSVWLRWVHEHRESKTWQASEVFRETAIQNVLQCKDRGVRNTALVTLIALHDERLRADLWKHVDASIKQLEVTDYGRITEQEISIYKCAEGQLYNTRVLDFDEAAGIKGTKGTSIQDQMAEIQLIRELAAKRQKEGKLSAKQKQVMEKELAAEKDTRDALRELYVTAEAKLDECRAMVAADAAGAFARPEILFEYCMPLTRSLLVSEEAARLFLAYRDACFEHSDDYLEELVGSNWLRVIGAHHRIPGWNDEFLDSALKRMFSLLNERAFVIDSGDDDDDEPVIFDDEIVGANHLTLLYPMIRIILKNSQRFSDNSRQDALQLLQSAIHKKFLRDRDVLNLPMEHYASMLFEHFSLDSGATNAFKATQQLFRLANDTNDIGDRVIGMVREILAYVGNEQPSVRGAALEILGAPHLLMRIVVELGIEQSISKEILVRIHVARHDPIEAVSEIAERLWIENHLQVKQVIGAMLVDECVSPSPLVRQSAAHAMVTFIEEHPNEMPAILTKFDETYKDLVLIREPIYDDVGRLQREAIDESDRRSGIGQTLVLLAGLCRQEEAEQLIRIVAPDGLSDRAQECRNELRNAAVETIRRHGAACMLRLLPLLEKLSDETPAQDDNRRQGLVVLLGTLAQYIDSTEKVKGIVARLVEALGTPSQTVQESVSRCLAPLVPKIRQDAKDLVSKLQWTLFEAETYGERRGAAYGIAGLMKGMGIIALKDTDLLGSIHKNMEDKKSPKHREGGLLALEILCCTIGKLFEPYILKALPSLLITFGDTDSNVRQSAEDTARAMMASMTVYGTKLVLPVLIVAIDDDSWRTKCAATELLGSMAFCAPRQLSACLPNIVPKLIEILADSSSKVQKSGEKALQQIARVVRNPEILGVTNQLMAGLLDPANKTSAALQAVLNTKFIHYIDAPSLALMMPIVRRAFEDRLSETRRVAAQIISNIYSLTENKDMEPYLAHMVPGLQRSLLDPVPEIRAVSARALGAVVSKSGGSTSENLRAQVIPWLKEKLVSPQSTVDRSGAAQGLCEVLAGAGTEQLEYVMPEIIHATESTDVSAETRDGYILMYIYLPMTFGDRFVPYLPQVVPPILKALADENEYVRASALKAGQRLISQYCSHARKLLLPQLQLALMDENWRIRYASVQLIGDFLFNISGITGKSTSSTADEDDTMGMEQAGKVIVRALGQKDRDRVLAGLYLTRSDVALVVRQAAGHVWKMVVSNTPRTLREVTKILFEMVVDSLASTCDERQQMGARCLGELVRKMGDKVINDILPVLDANQKSEEVAKRVGVAIALHEIIGNMSKEVTNHYLGAIVAPVRRAICDESELVREAAADTFTVLYHVVGNEALDEIICPLLEQLTPEQDHILAGLCDVMRQNSRSMLPYLLPKLTKPPVNVHALCSLASVSGDSLSRQLPKVLDALLAACETNDESDPMIESCEKVVIAVTDEDGIPVLVDYLIQKASQDENVPAAVLLSTFIAKSGVSLAEMAEEVLPGLLNLYTSPNPQIVDHAIAAAVALTQSMDQRELLSVLPVVKKAINIIVAGAKGQQIPGFTHPKSLQPLVVMLRESILQGQIEMKALAAECLGMVVKVSDVAALKAHVVNITGPLIRVLGDRFPANVKLPIIETLSKLLDKVDAMLRPFLPQLQSTFLKALQEPTSRPVRLAAGGALARLLKLHPKPEATMTELLKLLATSTDQQLIESSLATARALIATCGQKMSPTTIDEIYRVTELIYSENVENPTELDASLTACSGALLGETIAQKSDWKTAQNCVLSGIESPSTSARGRQAKACALQQLCSSNGDELWASEANSACRSAFTSAFTSPDPIVASAALRGASHVLKVSIDRDLMSAVARSLNHASTDVRKTAGIALGHVGHSADLPNDILKLIVPQLINGCKESNSQVRAASELALVHALKMTQNEDRFEAYRNTLEGVVQRNLDETRVSLARVVRAGDVDLEPLDTILNVY.
For Research Use Only | Not For Clinical Use.
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