Mouse Anti-C. elegans PGL3 Antibody (CBMOAB-08335HCB)

Cat: CBMOAB-08335HCB

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Size:
Conjugate:
	Inquiry

Specifications
Application Information
Target

Specifications

Host species	Mouse (Mus musculus)
Species Reactivity	C. elegans (Caenorhabditis elegans)
Clone	MO08335HB
Specificity	This antibody binds to C. elegans PGL3.
Format	Liquid or Lyophilized
Storage	Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use
Purity	> 90% was determined by SDS-PAGE
Purification	Purified with Protein A or G affinity chromatography

Application Information

Application	WB, ELISA
Application Notes	ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

Introduction	Guanyl-specific endoribonuclease which cleaves the phosphodiester bond in single-stranded RNA between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotide, resulting in the formation of a corresponding 2',3'-cyclic phosphate intermediate (PubMed:26787882). P-granule component involved in germline development (PubMed:15238518, PubMed:19372764, PubMed:24746798). Together with the P-granule component pgl-1, is involved in the formation of P-granules (PubMed:21402787, PubMed:24746798, PubMed:27594427). Together with pgl-1, probably recruits other granule components such as pos-1, mex-3 and glh-1, and RNA to P-granules (PubMed:21402787, PubMed:27594427). In vitro, binds mRNA; this interaction is required for the formation of liquid-like droplets that resemble P-granules (PubMed:27594427). Most likely recruits pgl-1 into P-granules during autophagy (PubMed:19167332). Associates with adapters such as sepa-1 and is required for the accumulation and degradation of P-granules by autophagy in somatic cells (PubMed:19167332, PubMed:24140420, PubMed:28806108). This ensures exclusive localization of the P-granules in germ cells (PubMed:19167332, PubMed:28806108). In addition, may act redundantly with pgl-1 to protect germ cells from excessive germline apoptosis during normal oogenesis and development of the two gonadal arms (PubMed:26598553). This may in part be through regulating the localization of sir-2.1 which is involved in germ cell apoptosis (PubMed:26598553). May protect somatic cells from excessive apoptosis during normal development (PubMed:27650246).
Product Overview	Mouse Anti-C. elegans PGL3 Antibody is a mouse antibody against PGL3. It can be used for PGL3 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative Names	PGL-3; Protein PGL-3, isoform a; pgl-3
UniProt ID	G5EBV6
Protein Refseq	The length of the protein is 693 amino acids long. The sequence is show below: MEANKRQIVEVDGIKSYFFPHLAHYLASNDELLVNNIAQANKLAAFVLGATDKRPSNEEIAEMILPNDSSAYVLAAGMDVCLILGDDFRPKFDSGAEKLSQLGQAHDLAPIIDDEKKISMLARKTKLKKSNDAKILQVLLKVLGAEEAEEKFVELSELSSALDLDFDVYVLAKLLGFASEELQEEIEIIRDNVTDAFEACKPLLKKLMIEGPKIDSVDPFTQLLLTPQEESIEKAVSHIVARFEEASAVEDDESLVLKSQLGYQLIFLVVRSLADGKRDASRTIQSLMPSSVRAEVFPGLQRSVFKSAVFLASHIIQVFLGSMKSFEDWAFVGLAEDLESTWRRRAIAELLKKFRISVLEQCFSQPIPLLPQSELNNETVIENVNNALQFALWITEFYGSESEKKSLNQLQFLSPKSKNLLVDSFKKFAQGLDSKDHVNRIIESLEKSSSSEPSATAKQTTTSNGPTTVSTAAQVVTVEKMPFSRQTIPCEGTDLANVLNSAKIIGESVTVAAHDVIPEKLNAEKNDNTPSTASPVQFSSDGWDSPTKSVALPPKISTLEEEQEEDTTITKVSPQPQERTGTAWGSGDATPVPLATPVNEYKVSGFGAAPVASGFGQFASSNGTSGRGSYGGGRGGDRGGRGAYGGDRGRGGSGDGSRGYRGGDRGGRGSYGEGSRGYQGGRAGFFGGSRGGS.

For Research Use Only | Not For Clinical Use.

Online Inquiry