AibGenesis™ Mouse Anti-C. elegans sas-1 Antibody (CBMOAB-58933FYC)
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- Specifications
- Application Information
- Target
- Relate Reference Data
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | C. elegans (Caenorhabditis elegans) |
| Clone | MO58933FYC |
| Specificity | This antibody binds to C. elegans sas-1. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
| Cellular Localization | Cytoskeleton |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Product Overview | Mouse Anti-C. elegans sas-1 (clone MO58933FYC) Antibody (CBMOAB-58933FYC) is a mouse antibody against sas-1. It can be used for sas-1 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Protein Y111B2A.24; sas-1 |
| UniProt ID | Q9BHL0 |
| Protein Refseq | The length of the protein is 570 amino acids long. The sequence is show below: MKPRRAPVIVKAFDENFQRPTEERDPYDDVQVEKVLKDLSFIRIKLDQIVLPDINPFIQKLHNYREFFGFQLEYTFPCLDAVSKSLKTTIRIPEKEVDIMKIVFNHRRIVLLNMHNDQIEHWQKSQLTIHLLVNMIVNGRQTTRPLAKCHVKLAELVRPPYLISRDFDFVGDDFEGTAELRIDLGSRVQSLTEKLTNLRNERSFDDNTFIVSDRQVGRRTRSRSSSRCRAHSSQSLGSATKDSARASASSSAAPSEIHKRSQSVTSIPAASTSTTTPTPILRPSTLQIPAQSYVAPPRPRIGSSASSMGSDSVFVRDPPTAQGAPPRVFEFPDSTESQFRERTHRTVTESYGASTVADTVEEVSRHGGKYYLEVTVHQATGLPPIEDESGCLASPSTLVSILGRDGDLRSPVFRNSRNPNWNFMARFALSSERRNLVVKVIHRGALLDLPLGFVSIPLPTPNFSRSVYEMTDITKMAAKYTHQVPMLTISVEKVRNIDDDDDDEENNHHYEQFRKRAHSSRSTQSSPPPPSAQYLLRIESPPILESSEVIKERMQRCMADLENMMRGINK. |
Relate Reference Data
IF
Figure 1 SAS-1 is a C2 domain containing protein that localizes to centrioles. Immunostainings of a GFP-SAS-1 embryo for GFP (yellow) and IFA (magenta).
IF
Figure 2 SAS-1 is a stable centriolar component recruited very early after fertilization independently of other centriolar proteins. Embryos stained for GFP to reveal GFP-SAS-1 (yellow) and IFA (magenta). DNA is in cyan. Note that a GFP focus was not detected in some embryos, perhaps due to a very weak signal. However, some embryos exhibit just one very bright signal, supporting a bona fide asymmetry of SAS-1 distribution between the two sperm centrioles. Note that the embryo in B is in telophase of the one-cell stage.
IP
Figure 3 Overexpression of SAS-1 in human cells reveals microtubule binding and stabilization activities. Immunoprecipitation using the GFP nanotrap of the indicated cell lysates probed with the indicated antibodies. In = Input = 140 µg, FT = flow through = 140 µg, IP = immunoprecipitation = 20% of input. We used 2 mg of protein lysate for GFP, 10 mg for SAS-1-P419S-GFP, and 22 mg for SAS-1-GFP to obtain sufficient material.