Anti-H3K27ac Antibody (Cat MO-MMB-0700), Rabbit IgG
Cat: MO-MMB-0700
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
- Product List
- Specifications
- Application Information
- Target
| Sub Cat | Clonality | Species Reactivity | Application | Clone | Conjugate | Size | |
| MO-MMB-0700 | Polyclonal | ChIP, ChIP-Seq, WB, DB | 100 µg | ||||
| MO-DKB-0252RA | Polyclonal | ELISA, IF, IG, WB | 50 µg | ||||
| MO-MMB-0699 | Polyclonal | Budding Yeast, Human (Homo sapiens) | ChIP, ChIP-Seq, Native ChIP, WB, IF, IHC(P), PLA | 100 µg |
Specifications
| Host species | Rabbit (Oryctolagus cuniculus) |
| Species Reactivity | Budding Yeast, Human (Homo sapiens), A. thaliana (Arabidopsis thaliana); Mouse (Mus musculus); Rat (Rattus norvegicus) |
| Immunogen | This Histone H3 acetyl Lys27 antibody is raised against peptides including the acetyl lysine 27 of histone H3. |
| Format | Liquid or Lyophilized |
| Buffer | 30% Glycerol |
| Regulatory Status | For Research Use Only |
| Shipping | Gel Pack |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
Application Information
| Application | ChIP, ChIP-Seq, WB, DB |
| Application Notes | ChIP: 3 µL per ChIP ChIP-Seq: 5 µL each WB: 1:1000-1:5000 The optimal dilution should be determined by the end user. |
Target
| Introduction | Histone H3 is one of the core components of nucleosomes. The nucleosome is the smallest subunit of chromatin and consists of 147 DNA base pairs wrapped around the core histone octamer (histone H2A, histone H2B, histone H3, and histone H4). Histone H1 is a linking histone that exists at the interface between the nucleosome core and DNA entry/exit points; it is responsible for building higher-order chromatin structures. Chromatin undergoes a variety of chemical modifications, including post-translational modifications of histones and methylation of cytosine residues in DNA. The reported histone modifications include acetylation, methylation, phosphorylation, ubiquitination, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. |
| Alternative Names | H3K27ac; Histone H3K27ac |
For Research Use Only | Not For Clinical Use.
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