Cat: MO-AB-10608Y
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- Product Details
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | O. mykiss (Oncorhynchus mykiss) |
| Clone | MO10608Y |
| Specificity | This antibody binds to O. mykiss AGO2. |
| Format | Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include ago2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by ago2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. |
| Product Overview | This product is a mouse antibody against AGO2. It can be used for AGO2 detection in Western Blot and Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Protein argonaute-2; Argonaute2; EC 3.1.26.n2; Eukaryotic translation initiation factor 2C 2; Protein slicer; eif2c2; ago2 |
| UniProt ID | A0A060XJA5 |
| Protein Refseq | The length of the protein is 873 amino acids long. The sequence is show below: MYSSGAAGAAEMLEPPHSSGSIGSDPPSPPVPEYVFKPPSRPDFGTMGRTIKLQANFFEMEIPKLEVYHYDIDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGREKVELEVTIPGEGKDRSFKVAIKWVSCVSLQALQEALSGRLPNIPFETIQALDVVMRHLPSMRYTPVGRSFFTPSEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLILRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSANFNNDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRQNKAIATPIQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNVQKTTPQTLSNLCLKINVKLGGVNNILLPQGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQDIIQDLATMVRELLIQFYKSTRFKPTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDQQALAKAVQIHQDTLRTMYFA. |
See other products for " AGO2 "
| CBMOAB-1842FYC | Mouse Anti-Arabidopsis AGO2 Antibody (CBMOAB-1842FYC) |
| CBMOAB-00766FYA | Mouse Anti-D. melanogaster Ago2 Antibody (CBMOAB-00766FYA) |
| MO-AB-42914W | Mouse Anti-Hamsters AGO2 Antibody (MO-AB-42914W) |
| MO-AB-07126R | Mouse Anti-Cattle AGO2 Antibody (MO-AB-07126R) |
| MO-DKB-0027RA | Rabbit Anti-AGO2 Antibody (MO-DKB-0027RA) |
| MO-AB-50593W | Mouse Anti-Marmoset AGO2 Antibody (MO-AB-50593W) |
| MO-AB-01290H | Mouse Anti-Frog AGO2 Antibody (MO-AB-01290H) |
| MO-AB-23623R | Mouse Anti-Pig AGO2 Antibody (MO-AB-23623R) |
| MO-AB-23622R | Mouse Anti-Pig AGO2 Antibody (MO-AB-23622R) |
| MO-AB-07113Y | Mouse Anti-Rabbit AGO2 Antibody (MO-AB-07113Y) |
For Research Use Only | Not For Clinical Use.