Mouse Anti-Arabidopsis AGO2 Antibody (CBMOAB-1842FYC)


Cat: CBMOAB-1842FYC
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityA. thaliana (Arabidopsis thaliana)
CloneMO1842FC
SpecificityThis antibody binds to Arabidopsis AGO2.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationNucleus; Cytosol

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionAGO proteins may be Slicer nucleases, and consistent with this idea, human AGO2 possesses a DDH motif that is critical for Slicer activity and is functionally equivalent to the catalytic metal-coordination triad DDE of RNase H.
Product OverviewMouse Anti-Arabidopsis AGO2 Antibody is a mouse antibody against AGO2. It can be used for AGO2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesArgonaute 2, RISC Catalytic Component; Argonaute RISC Catalytic Component 2; Cancer Susceptibility Candidate 7 (Non-Protein Coding); Eukaryotic Translation Initiation Factor 2C, 2; PAZ Piwi Domain Protein; Protein Slicer; EIF2C2; PPD; Eukaryotic Translation Initiation Factor 2C 2; Long Intergenic Non-Protein Coding RNA 980; Cancer Susceptibility Candidate 7; Protein Argonaute-2
UniProt IDQ9SHF3
Protein RefseqThe length of the protein is 1014 amino acids long. The sequence is show below: MERGGYRGGRGDGRGRGGRGYGGGGGGGEQGRDRGYGGGEQGRGRGSERGGGNRGQGRGEQQDFRSQSQRGPPPGHGGRGTTQFQQPRPQVAPQPSQAPASYAGSVGGVAGRGAWGRKPQVPSDSASPSTSTTVVSEPVRVAEVMNLKPSVQVATSDRKEPMKRPDRGGVVAVRRVNLYVNHYKVNFNPESVIRHYDVEIKGEIPTKKVSRFELAMVRDKVFTDNPDEFPLAMTAYDGQKNIFSAVELPTGSYKVEYPKTEEMRGRSYTFTIKQVNVLKLGDLKEYMTGRSSFNPRDVLQGMDVVMKEHPSKCMITVGKSFFTRETEPDEDFRFGVIAAKGYRHTLKPTAQGLSLCLDYSVLAFRKAMSVIEYLKLYFNWSDMRQFRRRDVEEELIGLKVTVNHRKNKQKLTIVGLSMQNTKDIKFDLIDQEGNEPPRKTSIVEYFRIKYGRHIVHKDIPCLDLGKNGRQNFVPMEFCDLVEGQIYPKDNLDKDSALWLKKLSLVNPQQRQRNIDKMIKARNGPSGGEIIGNFGLKVDTNMTPVEGRVLKAPSLKLAERGRVVREEPNPRQNNQWNLMKKGVTRGSIVKHWAVLDFTASERFNKMPNDFVDNLIDRCWRLGMQMEAPIVYKTSRMETLSNGNAIEELLRSVIDEASRKHGGARPTLVLCAMSRKDDGYKTLKWIAETKLGLVTQCFLTGPATKGGDQYRANLALKMNAKVGGSNVELMDTFSFFKKEDEVMFIGADVNHPAARDKMSPSIVAVVGTLNWPEANRYAARVIAQPHRKEEIQGFGDACLELVKAHVQATGKRPNKIVIFRDGVSDAQFDMVLNVELLDVKLTFEKNGYNPKITVIVAQKRHQTRFFPATNNDGSDKGNVPSGTVVDTKVIHPYEYDFYLCSHHGGIGTSKPTHYYTLWDELGFTSDQVQKLIFEMCFTFTRCTKPVSLVPPVYYADMVAFRGRMYHEASSREKNFKQPRGASTSAASLASSLSSLTIEDKAIFKLHAELENVMFFV.

Reference

Reference1. Baumberger, N., & Baulcombe, D. C. (2005). Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs. Proceedings of the National Academy of Sciences, 102(33), 11928-11933.
2. Ebhardt, H. A., Tsang, H. H., Dai, D. C., Liu, Y., Bostan, B., & Fahlman, R. P. (2009). Meta-analysis of small RNA-sequencing errors reveals ubiquitous post-transcriptional RNA modifications. Nucleic acids research, 37(8), 2461-2470.
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For Research Use Only | Not For Clinical Use.
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