Mouse Anti-AGO2 Antibody (CBMOAB-1842FYC)


Cat: CBMOAB-1842FYC
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Lot Number

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  • Specifications
  • Application Information
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Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-1842FYC Monoclonal A. thaliana (Arabidopsis thaliana), Cattle (Bos taurus), Dog (Canis lupus familiaris), Ferret (Mustela Putorius Furo), Frog (Xenopus laevis), Hamsters (Cricetinae), Marmoset, O. mykiss (Oncorhynchus mykiss), Pig (Sus scrofa), Rabbit (Oryctolagus cuniculus), Rhesus (Macaca mulatta), Rice (Oryza), Silkworm (Bombyx mori), Zebrafish (Danio rerio) WB, ELISA MO1842FC 100 µg
CBMOAB-35323FYA Monoclonal Rhesus (Macaca mulatta) WB, ELISA MO35323FYA 100 µg
CBMOAB-65207FYA Monoclonal Zebrafish (Danio rerio) WB, ELISA MO65207FYA 100 µg
CBMOAB-18540FYB Monoclonal Rice (Oryza) WB, ELISA MO18540FYB 100 µg
MO-AB-28889W Monoclonal Dog (Canis lupus familiaris) WB, ELISA MO28889W 100 µg
MO-AB-34346W Monoclonal Ferret (Mustela Putorius Furo) WB, ELISA MO34346W 100 µg
MO-AB-42914W Monoclonal Hamsters (Cricetinae) WB, ELISA MO42914W 100 µg
MO-AB-50593W Monoclonal Marmoset WB, ELISA MO50593W 100 µg
MO-AB-68777W Monoclonal Silkworm (Bombyx mori) WB, ELISA MO68777W 100 µg
MO-AB-07126R Monoclonal Cattle (Bos taurus) WB, ELISA MO07126R 100 µg
MO-AB-23623R Monoclonal Pig (Sus scrofa) WB, ELISA MO23623R 100 µg
MO-AB-01290H Monoclonal Frog (Xenopus laevis) WB, ELISA MO01290C 100 µg
MO-AB-07113Y Monoclonal Rabbit (Oryctolagus cuniculus) WB, ELISA MO07113Y 100 µg
MO-AB-10608Y Monoclonal O. mykiss (Oncorhynchus mykiss) WB, ELISA MO10608Y 100 µg
MO-DKB-0027RA Polyclonal A. thaliana (Arabidopsis thaliana) IP, WB 50 µg
MO-DKB-03978W Polyclonal A. thaliana (Arabidopsis thaliana) WB 100 µg

Specifications

Host speciesMouse (Mus musculus)
Species ReactivityA. thaliana (Arabidopsis thaliana), Cattle (Bos taurus), Dog (Canis lupus familiaris), Ferret (Mustela Putorius Furo), Frog (Xenopus laevis), Hamsters (Cricetinae), Marmoset, O. mykiss (Oncorhynchus mykiss), Pig (Sus scrofa), Rabbit (Oryctolagus cuniculus), Rhesus (Macaca mulatta), Rice (Oryza), Silkworm (Bombyx mori), Zebrafish (Danio rerio)
CloneMO1842FC
SpecificityThis antibody binds to Arabidopsis AGO2.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationNucleus; Cytosol

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionAGO proteins may be Slicer nucleases, and consistent with this idea, human AGO2 possesses a DDH motif that is critical for Slicer activity and is functionally equivalent to the catalytic metal-coordination triad DDE of RNase H.
Product OverviewMouse Anti-Arabidopsis AGO2 Antibody is a mouse antibody against AGO2. It can be used for AGO2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesArgonaute 2, RISC Catalytic Component; Argonaute RISC Catalytic Component 2; Cancer Susceptibility Candidate 7 (Non-Protein Coding); Eukaryotic Translation Initiation Factor 2C, 2; PAZ Piwi Domain Protein; Protein Slicer; EIF2C2; PPD; Eukaryotic Translation Initiation Factor 2C 2; Long Intergenic Non-Protein Coding RNA 980; Cancer Susceptibility Candidate 7; Protein Argonaute-2
UniProt IDQ9SHF3
Protein RefseqThe length of the protein is 1014 amino acids long. The sequence is show below: MERGGYRGGRGDGRGRGGRGYGGGGGGGEQGRDRGYGGGEQGRGRGSERGGGNRGQGRGEQQDFRSQSQRGPPPGHGGRGTTQFQQPRPQVAPQPSQAPASYAGSVGGVAGRGAWGRKPQVPSDSASPSTSTTVVSEPVRVAEVMNLKPSVQVATSDRKEPMKRPDRGGVVAVRRVNLYVNHYKVNFNPESVIRHYDVEIKGEIPTKKVSRFELAMVRDKVFTDNPDEFPLAMTAYDGQKNIFSAVELPTGSYKVEYPKTEEMRGRSYTFTIKQVNVLKLGDLKEYMTGRSSFNPRDVLQGMDVVMKEHPSKCMITVGKSFFTRETEPDEDFRFGVIAAKGYRHTLKPTAQGLSLCLDYSVLAFRKAMSVIEYLKLYFNWSDMRQFRRRDVEEELIGLKVTVNHRKNKQKLTIVGLSMQNTKDIKFDLIDQEGNEPPRKTSIVEYFRIKYGRHIVHKDIPCLDLGKNGRQNFVPMEFCDLVEGQIYPKDNLDKDSALWLKKLSLVNPQQRQRNIDKMIKARNGPSGGEIIGNFGLKVDTNMTPVEGRVLKAPSLKLAERGRVVREEPNPRQNNQWNLMKKGVTRGSIVKHWAVLDFTASERFNKMPNDFVDNLIDRCWRLGMQMEAPIVYKTSRMETLSNGNAIEELLRSVIDEASRKHGGARPTLVLCAMSRKDDGYKTLKWIAETKLGLVTQCFLTGPATKGGDQYRANLALKMNAKVGGSNVELMDTFSFFKKEDEVMFIGADVNHPAARDKMSPSIVAVVGTLNWPEANRYAARVIAQPHRKEEIQGFGDACLELVKAHVQATGKRPNKIVIFRDGVSDAQFDMVLNVELLDVKLTFEKNGYNPKITVIVAQKRHQTRFFPATNNDGSDKGNVPSGTVVDTKVIHPYEYDFYLCSHHGGIGTSKPTHYYTLWDELGFTSDQVQKLIFEMCFTFTRCTKPVSLVPPVYYADMVAFRGRMYHEASSREKNFKQPRGASTSAASLASSLSSLTIEDKAIFKLHAELENVMFFV.

Reference

Reference1. Baumberger, N., & Baulcombe, D. C. (2005). Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs. Proceedings of the National Academy of Sciences, 102(33), 11928-11933.
2. Ebhardt, H. A., Tsang, H. H., Dai, D. C., Liu, Y., Bostan, B., & Fahlman, R. P. (2009). Meta-analysis of small RNA-sequencing errors reveals ubiquitous post-transcriptional RNA modifications. Nucleic acids research, 37(8), 2461-2470.
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