AibGenesis™ Mouse Anti-SSL2 Antibody (CBMOAB-04241CR)


Cat: CBMOAB-04241CR

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Lot Number

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  • Specifications
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Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-04241CR Monoclonal Yeast, A. thaliana (Arabidopsis thaliana), Fruit fly (Drosophila melanogaster) WB, ELISA MO04241CR 100 µg
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CBMOAB-32029FYA Monoclonal Fruit fly (Drosophila melanogaster) WB, ELISA MO32029FYA 100 µg
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CBMOAB-41822FYC Monoclonal A. thaliana (Arabidopsis thaliana) WB, ELISA MO41822FC 100 µg
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityYeast, A. thaliana (Arabidopsis thaliana), Fruit fly (Drosophila melanogaster)
CloneMO04241CR
SpecificityThis antibody binds to Yeast SSL2.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationOther locations; Nucleus; Cytosol

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/SSL2, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/SSL2 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription (PubMed:8269516, PubMed:7693549, PubMed:7961739, PubMed:8202161, PubMed:7813015, PubMed:8631896). XPB/SSL2 acts as a double-stranded DNA translocase promoting DNA opening by tracking along the nontemplate promoter strand, rotating and inserting DNA into the Pol II active site cleft, leading to DNA unwinding. May also use this translocase mechanism during DNA repair rather than physically wedging open damaged DNA (PubMed:25775526). (From uniprot, under CC BY 4.0)
Product OverviewMouse Anti-Yeast SSL2 Antibody is a mouse antibody against SSL2. It can be used for SSL2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesDNA repair helicase RAD25; EC 3.6.4.12; General transcription and DNA repair factor IIH subunit RAD25; TFIIH subunit RAD25; Suppressor of stem-loop mutation 2; SSL2; LOM3 RAD25 UVS112; YIL143C
UniProt IDQ00578
Protein RefseqThe length of the protein is 843 amino acids long. The sequence is show below: MTDVEGYQPKSKGKIFPDMGESFFSSDEDSPATDAEIDENYDDNRETSEGRGERDTGAMVTGLKKPRKKTKSSRHTAADSSMNQMDAKDKALLQDTNSDIPADFVPDSVSGMFRSHDFSYLRLRPDHASRPLWISPSDGRIILESFSPLAEQAQDFLVTIAEPISRPSHIHEYKITAYSLYAAVSVGLETDDIISVLDRLSKVPVAESIINFIKGATISYGKVKLVIKHNRYFVETTQADILQMLLNDSVIGPLRIDSDHQVQPPEDVLQQQLQQTAGKPATNVNPNDVEAVFSAVIGGDNEREEEDDDIDAVHSFEIANESVEVVKKRCQEIDYPVLEEYDFRNDHRNPDLDIDLKPSTQIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVLCTSSVSVMQWRQQFLQWCTLQPENCAVFTSDNKEMFQTESGLVVSTYSMVANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVSTIAAHAKLGLTATLVREDDKIGDLNFLIGPKLYEANWMELSQKGHIANVQCAEVWCPMTAEFYQEYLRETARKRMLLYIMNPTKFQACQFLIQYHERRGDKIIVFSDNVYALQEYALKMGKPFIYGSTPQQERMNILQNFQYNDQINTIFLSKVGDTSIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVSKDTQEMYYSTKRQAFLVDQGYAFKVITHLHGMENIPNLAYASPRERRELLQEVLLKNEEAAGIEVGDDADNSVGRGSNGHKRFKSKAVRGEGSLSGLAGGEDMAYMEYSTNKNKELKEHHPLIRKMYYKNLKK.
For Research Use Only | Not For Clinical Use.
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