ArcticExpress (DE3) pRARE2 competent cells (MOFY-0822-FY511)


Cat: MOFY-0822-FY511
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Specifications

DescriptionArcticExpress (DE3) pRARE2competent cells
It can express low temperature-adapted chaperone proteins Cpn10 and Cpn60, reduce the formation of inclusion bodies, and increase the expression and biological activity of soluble proteins.
Gene TypesE. coli B F- ompT hsdS (rB- mB-) dcm+ TetR gal λ (DE3) endA Hte [cpn10cpn60 GentR] pRARE2 (CamR)
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×108 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. The ArcticExpress (DE3) pRARE2 strain carries the pRARE2 plasmid. Except for the recovery medium without antibiotics, the other medium and culture medium should contain 34 µg/ml lvmycin and 40ug/ml qingdamycin to prevent the plasmid lost.
3. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter.
4. ArcticExpress (DE3) pRARE2competent cells are resistant to tetracycline, gentamicin, and chloramphenicol, and cannot be used for the transformation of plasmids with resistance to tetracycline, gentamicin, and chloramphenicol.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
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