ArcticExpress (DE3) competent cells (MOFY-0822-FY512)


Cat: MOFY-0822-FY512
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Specifications

DescriptionArcticExpress (DE3)competent cells
ArcticExpress (DE3) is derived from E. coli B and is a Lon and OmpT protease-deficient strain that promotes the stability of expressed proteins.
Gene TypesE. coli B F- ompT hsdS (rB- mB-) dcm+ TetR gal λ (DE3) endA Hte [cpn10cpn60 GentR]
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×108 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. Except for the recovery medium without antibiotics, the other medium and culture medium should contain 40ug/ml gentamicin to prevent the loss of plasmids.
3. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter.
4. ArcticExpress (DE3)competent cells are resistant to tetracycline and gentamicin and cannot be used for the transformation of plasmids with tetracycline and gentamicin resistance.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
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