C58C1 electro competent cells (MOFY-0822-FY549)
Cat: MOFY-0822-FY549
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Specifications
| Description | A. rhizogenes is a Gram-negative soil bacterium of the genus agrobacterium of the family Rhizobiaceae, which is capable of infecting most dicotyledonous plants and a few monocotyledonous plants as well as individual gymnosperms. C58C1 Agrobacterium rhizogenes strain contains pRiA4b agrobacterium-type Ri plasmid, which has a wide host range (Rosaceae, Oleander, Fabaceae, Solanaceae, Scutellaria, tobacco, etc.), streptomycin, rifampicin, etc. resistance. |
| Gene Types | Agrobacterium rhizogenes (strR,lifR ) pRiA4b (agropine type) |
| Size | 50 µL/vial |
| QC result | Transformation efficiency of pCAMBIA2301 plasmid (kanamycin resistance) detection:>105 cfu/μg DNA; Transformation efficiency of pCAMBIA2301-ZH plasmid (size:40 kb) detection:>5×103 cfu/μg DNA. |
| Note | 1. When the plasmid is added, the volume should not be greater than 1/10 of the competent volume; if the plasmid is impure or there is organic pollution such as ethanol, the transformation efficiency will drop sharply; if the plasmid is doubled, the transformation efficiency will drop by an order of magnitude. 2. Air bubbles should not be mixed in the electroporation of competitive cells. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are finally used for plating. 3. When the density of positive clones on the plate is too large, due to insufficient nutrition, the growth of positive clones will slow down and the colonies will become smaller. In order to obtain large colonies, the amount of plasmids or the amount of bacteria finally used for plating should be reduced. 4. C58C1 is resistant to streptomycin and rifampicin, and cannot be used for the transformation of plasmids with streptomycin resistance or rifampicin resistance. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
Related Products
| MOFY-0822-FY548 | C58C1 competent cells (MOFY-0822-FY548) |
For Research Use Only | Not For Clinical Use.
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