EHA105(pSoup) competent cells (MOFY-0822-FY533)


Cat: MOFY-0822-FY533
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Specifications

DescriptionThe EHA105 strain is transformed from the EHA101 strain and has a C58 background. The nuclear gene contains a screening tag-rifampicin resistance gene rif, and pSoup is the EHA105 (pSoup) strain. It can replicate in Bacillus, and at the same time endow the strain with tetracycline (tet) resistance, which is suitable for transgenic operations of rice, tobacco and other plants.
Gene TypesC58 (rifR) Ti pEHA105 (pTiBo542DT-DNA) Succinamopine (pSoup-tetR)
Size100 µL/vial
QC resultTransformation efficiency of pGs2 plasmid (kanamycin resistance) detection:>104 cfu/μg DNA
Note1. When the plasmid is added, the volume should not be greater than 1/10 of the competent volume; if the plasmid is impure or there is organic pollution such as ethanol, the transformation efficiency will drop sharply; if the plasmid is doubled, the transformation efficiency will drop by an order of magnitude.
2. Transforming a high concentration of plasmid can correspondingly reduce the amount of bacteria finally used for plating. The EHA105 (pSoup) chemically transformed competitive cells produced by our company are resistant to tetracycline, but when they are transferred into the target plasmid to screen for positive clones, only Antibiotics resistant to the target plasmid need to be added, but tetracycline is not added.
3. When the density of positive clones on the plate is too large, due to insufficient nutrition, the growth of positive clones will slow down and the colonies will become smaller. In order to obtain large colonies, the amount of plasmid should be reduced.
4. The concentration of rifampicin should not be higher than 25 μg/ml. Too high concentration of rifampicin is not conducive to the growth of Agrobacterium and will reduce its growth rate and transformation efficiency.
5. The purpose of adding rifampicin to the medium is to prevent the growth of miscellaneous bacteria and to screen Agrobacterium; adding streptomycin or gentamicin according to the resistance of the strain used can prevent the loss of Ti plasmid, but streptomycin is not conducive to the growth of Agrobacterium. In transgenic operations, streptomycin or gentamicin is not considered when culturing Agrobacterium, and the probability of loss of Ti plasmid is extremely low (can be ignored).

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
Related Products
MOFY-0822-FY533EHA105(pSoup) competent cells (MOFY-0822-FY533)
MOFY-0822-FY534EHA105 competent cells (MOFY-0822-FY534)
For Research Use Only | Not For Clinical Use.
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