EPI300 chemically competent cells (MOFY-0822-FY477)
Cat: MOFY-0822-FY477
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | EPI300 cells contain a mutated trfA gene, the protein product expressed by this gene can promote the high-copy expansion of the plasmid containing the ori V replicon, and inducer I can induce the expression of the trfA gene |
| Gene Types | F - mcrA ∆ (mrr-hsdRMS-mcrBC) φ80dlacZ∆M15 ∆lacX74 recA1 endA1 araD139 ∆ (ara,leu)7697 galU galK λ - rpsL nupG trfA dhfr. |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 3×108 cfu/μg DNA |
| Note | Competent cells are suitable for slow thaw in ice. Add the target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
Related Products
| MOFY-0822-FY476 | EPI300 electro competent cells (MOFY-0822-FY476) |
For Research Use Only | Not For Clinical Use.
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