EPI400 chemically competent cells (MOFY-0822-FY475)
Cat: MOFY-0822-FY475
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | EPI400 is derived from EC100 strain. After deleting the pcnB gene that controls the plasmid copy number in the EC100 nuclear gene, an inducible promoter-driven pcnB gene is introduced, which is the EPI100 strain. The EPI400 strain can reduce the copy number of plasmids, especially suitable for the cloning of various unstable DNA or virulence genes. After adding WDEPI-inducer I, the plasmid yield can be increased to a normal state. |
| Gene Types | F- mcrA Δ (mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA pcnB4 dhfr |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 5×107 cfu/μg DNA |
| Note | 1. Competent cells should be thawed slowly in ice. Add the target DNA within 10 minutes of inserting it into ice. Do not keep it in ice for too long, as long-term storage will reduce the transformation efficiency. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
Related Products
| MOFY-0822-FY474 | EPI400 electro competent cells (MOFY-0822-FY474) |
For Research Use Only | Not For Clinical Use.
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