ER2566 competent cells (MOFY-0822-FY505)


Cat: MOFY-0822-FY505
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Specifications

DescriptionThe ER2566 strain is a protein-expressing prokaryotic strain with ultra-high transformation efficiency developed by NEB, derived from BL21. Lac promoter promotes the expression of downstream T7 RNA polymerase, which can be used for high-level protein expression of T7 promoter expression vectors (such as pET series). fhuA2 confers resistance to phage T1 in strain ER2566. At the same time ER2566 is a lon and ompT protease deficient strain.
Gene TypesF- λ- fhuA2 [lon] ompT lacZ::T7 gene1 gal sulA11 Δ (mcrC-mrr)114::IS10 R (mcr-73::miniTn10-TetS)2 R (zgb-210::Tn10) (TetS) endA1 [dcm]
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection>5×109 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Be gentle when mixing plasmids.
3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating.
4. During induction, IPTG concentration is optional (0.1-2mM can be used).
5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
For Research Use Only | Not For Clinical Use.
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