JM110 chemically competent cells (MOFY-0822-FY462)
Cat: MOFY-0822-FY462
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Specifications
| Description | JM110 strain has streptomycin sulfate resistance (StrR); it is a strain with deletion of methylation genes dam and dcm, and the extracted plasmid DNA can be cleaved by endonucleases sensitive to methylation of dam and dcm; the presence of lacIqlacZΔM15 It enables JM110 to perform blue-white screening, but the transformation efficiency is not high. Generally, it is not used for plasmid construction, but only for plasmid transformation. |
| Gene Types | rpsL (StrR) thr leu thi-1 lacY galK galT ara tonA tsx dam dcm supE44 Δ (lac-proAB) /F' [traD36 proAB lacIqlacZΔM15] |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 1×107 cfu/μg DNA |
| Note | 1. Competent cells should be slowly thawed in ice, and the target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids. 3. Transforming a high concentration of plasmid can correspondingly reduce the amount of bacteria that is finally used for plating. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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