MG1655 chemically competent cells (MOFY-0822-FY484)


Cat: MOFY-0822-FY484
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  • Specifications
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Specifications

DescriptionThe MG1655 strain is derived from W1485 and is a derivative of K12. It is an E. coli engineered strain that has undergone less modification and is closer to "WT-wild type". MG1655 has a standard appearance and shape, and can be used as a template for amplifying the WT gene of E. coli. It can also be used as a host strain for protein expression, but it does not contain nuclease endA1 mutation, and the content of nuclease in the body is high. Be sure to use it when extracting plasmids The protein removal solution in the plasmid extraction kit removes nuclease as much as possible to prevent the extracted plasmid from being degraded.
Gene TypesK12 F- lambda- ilvG- rfb-50 rph-1
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 0.5×108 cfu/μg DNA
Note1. Competent cells should be slowly thawed in ice. The target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Transforming high concentrations of plasmids or high-efficiency ligation products can correspondingly reduce the amount of bacteria that will be used for plating.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
For Research Use Only | Not For Clinical Use.
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