Mouse Anti-E. coli ada Antibody (CBMOAB-0038YC)


Cat: CBMOAB-0038YC
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityE. coli (Escherichia coli )
CloneMO0038YC
SpecificityThis antibody binds to Escherichia coli K-12 ada.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionInvolved in the adaptive response to alkylation damage in DNA caused by alkylating agents. Repairs O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme (Cys-321). Also specifically repairs the Sp diastereomer of DNA methylphosphotriester lesions by the same mechanism, although the methyl transfer occurs onto a different cysteine residue (Cys-38). Cannot demethylate the other diastereomer, Rp-methylphosphotriester. This is a suicide reaction: the enzyme is irreversibly inactivated.
Product OverviewMouse Anti-E. coli ada Antibody is a mouse antibody against ada. It can be used for ada detection in Western Blot and Enzyme-Linked Immunosorbent Assay.
Alternative NamesBifunctional transcriptional activator/DNA repair enzyme Ada; Regulatory protein of adaptive response; O6-methylguanine-DNA alkyltransferase; Methylphosphotriester-DNA methyltransferase; EC 2.1.1.63; EC 2.1.1.n11; ada; b2213 JW2201
UniProt IDP06134
Protein RefseqThe length of the protein is 354 amino acids long. The sequence is show below: MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRARHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTLEALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFPDSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER.
For Research Use Only | Not For Clinical Use.
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