OverExpress C43(DE3) competent cells (MOFY-0822-FY503)
Cat: MOFY-0822-FY503
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | OverExpress C43(DE3)competent cells Both OverExpress C43(DE3) and OverExpress C41(DE3) are derived from BL21(DE3), and their advantages are that they can express toxic or hydrophobic proteins efficiently |
| Gene Types | F-ompT hsdSB (rB- mB-) gal dcm (DE3) |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 5×108 cfu/μg DNA |
| Note | 1. Competent cells should be thawed on ice as much as possible. 2. Be gentle when mixing plasmids. 3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. 4. During induction, IPTG concentration is optional (0.1-2mM can be used). 5. In order to obtain the required amount of protein, the induction time, temperature and IPTG concentration need to be optimized by the experimenter. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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