TG1 chemically competent cells (MOFY-0822-FY469)
Cat: MOFY-0822-FY469
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | TG1 is derived from E. coli K-12 strain, which is currently the fastest growing E. coli strain for cloning. Cloning can be seen on the plate at 37°C for 7 hours. The main phage display strains can also be used for the construction of common plasmids. The existence of lacIqZΔM15 makes it suitable for experiments such as blue-white spot screening; however, it does not contain nuclease endA1 mutation, and the nuclease content in the body is relatively high. It is recommended to use when extracting plasmids Plasmid extraction kit to remove protein solution to remove a large number of nucleases in bacteria. |
| Gene Types | [F´traD36 proAB lacIqZΔM15] supE thi-1Δ (lac-proAB)Δ (mcrB-hsdSM)5 (rK-mK-) |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection>5×109 cfu/μg DNA |
| Note | 1. Competent cells should be slowly thawed in ice, and the target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids or ligation products. 3. Transforming high concentrations of plasmids or high-efficiency ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
For Research Use Only | Not For Clinical Use.
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