Mouse Anti-Chicken MOCS3 Antibody (MO-AB-02945Y)
Cat: MO-AB-02945Y
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- Product Details
Specifications
| Host species | Mouse (Mus musculus) |
| Species Reactivity | Chicken (Gallus gallus) |
| Clone | MO02945Y |
| Specificity | This antibody binds to Chicken MOCS3. |
| Format | Liquid or Lyophilized |
| Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
| Purity | > 90% was determined by SDS-PAGE |
| Purification | Purified with Protein A or G affinity chromatography |
| Cellular Localization | Cytosol; Other locations |
Application Information
| Application | WB, ELISA |
| Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
| Introduction | Plays a central role in 2-thiolation of mcmSU at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. |
| Product Overview | This product is a mouse antibody against MOCS3. It can be used for MOCS3 detection in Western Blot and Enzyme-Linked Immunosorbent Assay. |
| Alternative Names | Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; MOCS3; UBA4 |
| UniProt ID | E1C3M4 |
| Protein Refseq | The length of the protein is 468 amino acids long. The sequence is show below: MAGGAEAAWLSVEIEQRERELRGLREQLAAVLAEEHGGAAQQGAGAVDADFPAKLPPLPAQAALSPADILRYSRQLVLPELGVRGQLLLARSSVLVVGCGGLGCPLAQYLAAAGVGRLGLVDHDVVETSNLHRQVLHGEARRGFPKAASAAAALRQLNSTVQYVPYCGALTPRSALQLVQQYDVVADCSDNVPTRYLVNDACVLAGKPLVSGSALRLEGQLVVYNYHGGPCYRCLFPKPPPPETVTNCADGGVLGVVPGIMGCIQALEVLKIISGMGSSFNQFMLMFDAQEGRFRNIKLRPKKSDCAVCGDNPSVTCLQDYEAFCGSSATDKCRTLHLLSSKDRISVEEYKKLLDEQVPHVLLDVRPKVEVNICRLEHAVHIPLSKLEEKDEECMEYLEKIIFEEKQRTDGQTSFPVYVVCKLGNDSQKAVRILQELPVKEFGPVLAKDIKGGLMTWASRIDPTFPQY. |
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For Research Use Only | Not For Clinical Use.
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