Mouse Anti-O. mykiss MOCS3 Antibody (MO-AB-12101Y)
Cat: MO-AB-12101Y
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- Product Details
Specifications
Host species | Mouse (Mus musculus) |
Species Reactivity | O. mykiss (Oncorhynchus mykiss) |
Clone | MO12101Y |
Specificity | This antibody binds to O. mykiss MOCS3. |
Format | Liquid or Lyophilized |
Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
Purity | > 90% was determined by SDS-PAGE |
Purification | Purified with Protein A or G affinity chromatography |
Cellular Localization | Other locations; Cytosol |
Application Information
Application | WB, ELISA |
Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
Introduction | Plays a central role in 2-thiolation of mcmSU at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. |
Product Overview | This product is a mouse antibody against MOCS3. It can be used for MOCS3 detection in Western Blot and Enzyme-Linked Immunosorbent Assay. |
Alternative Names | Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; mocs3; uba4 |
UniProt ID | A0A060VTT7 |
Protein Refseq | The length of the protein is 480 amino acids long. The sequence is show below: MAEEVSCLKARLMEKEKEIVALKNKLALEKNSLIDLQLQEKVTTLPPLKLKATLSNEDIMRYSRQLILPEFGVQGQLNLSQTSVLVVGCGGLGCPLAQYLAAAGIGRVGLLDYDEVELSNLHRQVLHGEDSQGQAKAQSAAHAVRRLNSTVECIPYHLQLSPENALQLIQQYPPSQPWKKITASLLLDIIHLMYDIVADCSDNVPTRYLVNDACVLSGKPLVSASALRMEGQLTVYNYRGGPCYRCLYPKPPPPEMVTNCSDGGILGVVPGIMGCFQALEVLKIASGQGSSCGQQLLMFDAQDAKFRSIRLRSKQASCAVCGENPTVTHLVDYEKFCGSAATDKCRRLNLLSKDQRVTVQEYKSIVDNTAAHLLLDVRPLVEVDICHLPTSLNIPLSSLEDRKSEHIRVLKERIGRVKEEMLNESQVPVYVICKLGNDSQKAVQVLEKMSGSEVDFVTVKDICGGLMAWAQKIDPSFPQY. |
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For Research Use Only | Not For Clinical Use.
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