Mouse Anti-Elephant MOCS3 Antibody (MO-AB-00820L)


Cat: MO-AB-00820L
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityElephant (Loxodonta africana)
CloneMO00820L
SpecificityThis antibody binds to Elephant MOCS3.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationCytosol; Other locations

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionPlays a central role in 2-thiolation of mcmSU at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
Product OverviewThis product is a mouse antibody against MOCS3. It can be used for MOCS3 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesMolybdenum Cofactor Synthesis 3; Ubiquitin-Like Modifier Activating Enzyme 4; Molybdenum Cofactor Synthesis Protein 3; Molybdopterin Synthase Sulfurylase; MPT Synthase Sulfurylase; UBA4; UBA4, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); Adenylyltransferase And Sulfurtransferase MOCS3; UBA4, Ubiquitin-Activating Enzyme E1 Homolog
UniProt IDG3TPH1
Protein RefseqThe length of the protein is 460 amino acids long. The sequence is show below: MAARDEVLALRTEVAQREEELSSLKQRLAAALLEKQESESERLVLVSPLPPKAALSRDEILRYSRQLVLPELGVHGQLRLATASVLVVGCGGLGCPLAQYLAAAGVGRIGLVDYDVVEMSNLPRQVLHDEALAGQAKAFSAAAALRRLNSAVECVPYAQALTPATALDLVRRYDVVADCSDNVPTRYLVNDACVLAGRPLVSASALRFEGQITVYHYDGGPCYRCVFPQPPPAETVTNCADGGVLGVVTGVLGCLQALEVLKIAAGLGPSYSGSLLLFDALRGHFRCIRLRKRRPNCAACGERPTVTDLQDYEAFCGSSATDKCRSLQLLSPKERVSVTEYKQLLDSGTSHLLLDVRPQVEVDICRLPHALHIPLKRLERGDAESLKLLREAVRERKQGVQEGAAVPVYVICKLGNDSQKAVKILQSLTAAQELDSLTVQDVVGGLMAWAAKIDDTFPQY.
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For Research Use Only | Not For Clinical Use.
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