Rabbit Anti-HIF1A (AA 432-528) Antibody (Cat MO-DKB-03268W)


Cat: MO-DKB-03268W
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Specifications

Host speciesRabbit (Oryctolagus cuniculus)
Species ReactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Cattle (Bos taurus), Dog (Canis lupus familiaris), Guinea pig (Cavia porcllus), Primate, Frog (Xenopus), Zebrafish (Danio rerio)
SpecificityThis antibody is binds to Human HIF-1 alpha and has cross reactivity with Mouse, Rat, Cattle, Dog, Guinea pig, Primate, Frog, Zebrafish HIF-1 alpha.
ImmunogenThe HIF-1 α antibody was developed against a fusion protein, which forms a fusion protein with the internal sequence of human HIF-1 α (containing amino acids 432-528) [Uniprot# Q16665].
EpitopeAA 432-528
FormatLiquid or Lyophilized
BufferPBS
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
PurificationAffinity purified
Cellular LocalizationNucleus

Application Information

ApplicationWB, ELISA, GS, IB, IF, IHC, IHC-Fr, IHC-P, IP, ChIP, Dual ISH-IHC, KO
Application NotesWestern Blot: 1:500-1:1000
Simple Western: 1:100
Gel Super ShImmunofluorescence:t Assays 1:1-1:100
Immunoblotting
Immunocytochemistry/Immunofluorescence: 1:100-1:500
Immunohistochemistry: 1:100-1:500
Immunohistochemistry-Frozen: 1:100-1:500
Immunohistochemistry-Paraffin: 1:100-1:500
Immunoprecipitation: 1:1000
The optimal dilution should be determined by the end user.

Target

IntroductionThis gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
Product OverviewThis product is a Rabbit antibody against the HIF-1 alpha. It can be used for HIF-1 alpha detection in Western Blot, Enzyme-Linked Immunosorbent Assay, Gel Super Shift Assay, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Chromatin Immunoprecipitation, Dual In-situ Hybridization-Immunohistochemistry, Knockout Validated.
Alternative NamesHypoxia Inducible Factor 1 Alpha Subunit; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor); Class E Basic Helix-Loop-Helix Protein 78; Basic-Helix-Loop-Helix-PAS Protein MOP1; PAS Domain-Containing Protein 8; Member Of PAS Protein 1; HIF-1-Alpha; HIF1-ALPHA; BHLHe78; PASD8; MOP1;
Gene ID3091
UniProt IDQ16665
See other products for " HIF1A "
MO-AB-26318RMouse Anti-Pig HIF1A Antibody (MO-AB-26318R)
MO-AB-15986WMouse Anti-Chimpanzee HIF1A Antibody (MO-AB-15986W)
MO-AB-31117WMouse Anti-Dog hif1a Antibody (MO-AB-31117W)
MOFY-0722-FY346Rabbit Anti-HIF1a Antibody (MOFY-0722-FY346)
MO-AB-56725WMouse Anti-Marmoset HIF1A Antibody (MO-AB-56725W)
MO-AB-27164WMouse Anti-Chimpanzee HIF1A Antibody (MO-AB-27164W)
MO-AB-13660RMouse Anti-Cattle HIF1A Antibody (MO-AB-13660R)
MO-AB-45028WMouse Anti-Horse HIF1A Antibody (MO-AB-45028W)
MO-AB-15633YMouse Anti-Sheep HIF1A Antibody (MO-AB-15633Y)
MO-AB-04231HMouse Anti-Frog hif1a Antibody (MO-AB-04231H)
For Research Use Only | Not For Clinical Use.
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