Mouse Anti-TNF Antibody (AA 115-130)


Cat: MO-NAB-00379W
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Dog (Canis lupus familiaris), Cat (Felis catus), Rabbit (Oryctolagus cuniculus), Zebrafish (Danio rerio)
CloneNW0304
SpecificityThis antibody is binds to Human TNF and has cross reactivity with Mouse, Rat, Dog, Cat, Rabbit, Zebrafish.
ImmunogenHexadepeptide corresponding to aa115-130 of human TNF-α, which binds to thyroglobulin.
EpitopeThe epitope is in the AA 115-130 of TNF.
FormatLiquid or Lyophilized
Buffer10 mM PBS, 0.05% BSA.
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
PurificationProtein A purified
Cellular LocalizationCytoplasmic and extracellular (secreted)

Application Information

ApplicationFC, IF, IHC, IHC-P
Application NotesFlow Cytometry: 0.5-1ug/million cells
Immunofluorescence: 1-2ug/ml
Immunohistochemistry: Assay-dependent
Immunohistochemistry-Paraffin: 1-2ug/ml

Target

IntroductionTumor necrosis factor (TNF, cachexia or cachexia protein; once called tumor necrosis factor alpha or TNFα) is a cell signaling protein (cytokine) involved in systemic inflammation, and is one of the cytokines that constitute an acute phase response. It is mainly produced by activated macrophages, although it can be produced by many other cell types, such as T helper cells, natural killer cells, neutrophils, mast cells, eosinophils, and neurons. TNF is a member of the TNF superfamily and consists of various transmembrane proteins with homologous TNF domains.
Product OverviewThis product is a Mouse antibody against the AA 115-130 of the TNF. It can be used for TNF detection in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin.
Alternative NamesTNF; DIF; TNF-alpha; TNFA; TNFSF2; Tumour necrosis factor; TNF-α; tumor necrosis factor; TNLG1F; Tumor necrosis factor alpha
Gene ID7124(Human); 21926(Mouse)
UniProt IDP01375(Human); P06804(Mouse)
For Research Use Only | Not For Clinical Use.
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