Mouse Anti-Zebrafish cd4 Antibody (CBMOAB-69695FYA)


Cat: CBMOAB-69695FYA
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Specifications

Host speciesMouse (Mus musculus)
Species ReactivityZebrafish (Danio rerio)
CloneMO69695FYA
SpecificityThis antibody binds to Zebrafish cd4.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationOther locations; Plasma Membrane

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionCD4 (CD4 Molecule) is a Protein Coding gene. Diseases associated with CD4 include Okt4 Epitope Deficiency and Pilonidal Sinus. Among its related pathways are G-protein signaling N-RAS regulation pathway and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and enzyme binding.
Product OverviewMouse Anti-Zebrafish cd4 Antibody is a mouse antibody against cd4. It can be used for cd4 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesCD4 protein; cd
UniProt IDU6C9E1
Protein RefseqThe length of the protein is 476 amino acids long.
The sequence is show below: MLGLILIPLFITVLKAQESHEVIYAQVGGTVTIPRDKIERKDGNIKTQDIYVNWFLDSESTPTINRNPQSGSSKVKNARVSLSADFSLQISPVEESDFVIWRCEQHVLAGNYKKTYKLYKVSIPKVPALLVGGHLFLKYVKDVSSVNPSVTWISPKNEGCQENKTTKDTVLVTSVSTCHNGVWTCQLKYGNKKTEATTTVSVIDLAPSPADPIYTSISQSSTVSIPCALSSAIPWSVLNETLQGGSWSFTPLSEPRSPLSLLTLNVGSVVRWDLANGANFTDGKRVITNHNLSIQNLPVKETIRGVYKCSLKFNTKTISREVKVEVLKVSPSGSLKVFEGTRLNVTCSLGHMNTAGLEVKWTCASNCPPFNHKSPPHLSVLSFPNIRMQDKGLVKCELWKNSQKLTSAQLYLRVEKAPVDIWLCVAIGSGVVVFILLVAFAIIYIRRHKQMMMYRRRKTRFCCCNNNKQPKGFYKT.

Reference

Reference1. Yoon, S., Mitra, S., Wyse, C., Alnabulsi, A., Zou, J., Weerdenburg, E. M., ... & Bird, S. (2015). First demonstration of antigen induced cytokine expression by CD4-1+ lymphocytes in a poikilotherm: Studies in zebrafish (Danio rerio). PloS one, 10(6), e0126378.
2. Dee, C. T., Nagaraju, R. T., Athanasiadis, E. I., Gray, C., Fernandez del Ama, L., Johnston, S. A., ... & Hurlstone, A. F. (2016). CD4-transgenic zebrafish reveal tissue-resident Th2-and regulatory T cell-like populations and diverse mononuclear phagocytes. The Journal of Immunology, 197(9), 3520-3530.
3. Miyazawa, R., Matsuura, Y., Shibasaki, Y., Imamura, S., & Nakanishi, T. (2018). Cross-reactivity of monoclonal antibodies against CD4-1 and CD8α of ginbuna crucian carp with lymphocytes of zebrafish and other cyprinid species. Developmental & Comparative Immunology, 80, 15-23.
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Figure 1 Zebrafish CD4-1/CD4-2 gene distribution. Cell profile obtained from FACS sorted zf kidney and spleen cells. E = erythrocytes, L = lymphocytes, P = (hemopoietic) precursor cells and M/G = monocytes/granulocytes.

Figure 2 Zebrafish CD4-1 protein expression in transfected CHO cells and zebrafish tissues. Western blot analysis of zfCD4-1 protein.

For Research Use Only | Not For Clinical Use.
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