DH5α electro competent cells (MOFY-0822-FY459)


Cat: MOFY-0822-FY459
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DescriptionElectroporation of DH5α into competitive cells can only be used for electroshock transformation, not for heat shock transformation. DH5α strains are commonly used competitive cells in laboratories. The deletion of endonuclease (endA) improves the yield and quality of plasmid DNA; the recombinase-deficient type (recA) reduces the probability of homologous recombination of the inserted fragment and ensures the stability of the inserted DNA; the presence of lacZΔM15 makes DH5α available for blue, vitiligo screening.
Gene TypesF- φ80 lac ZΔM15 Δ (lacZYA-arg F) U169 endA1 recA1 hsdR17 (rk-,mk+) supE44λ- thi -1 gyrA96 relA1 phoA
Size50 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×1010 cfu/μg DNA
Note1. When adding DNA, the volume should not be greater than 1/10 of the competent volume.
2. When adding electric shock competent cells to the electric shock cup, avoid generating air bubbles, which will increase the risk of arc discharge.
3. When the DNA is impure or contaminated with salt, ethanol, protein and buffer, the transformation efficiency drops sharply.
4. The ions in the shock cup can increase the conductance of the solution, increasing the risk of electric current and arcing in solutions containing cells and DNA.
5. If you want to transform large plasmids or want to obtain higher transformation efficiency, it is recommended to use a high-purity plasmid extraction kit to extract the plasmids. Double the size of the plasmid, and the transformation efficiency drops by an order of magnitude.
6. For ligation product transformation, ethanol-precipitate the DNA before transformation and resuspend the product with an appropriate amount of TE buffer (10 mM Tris HCl, pH7.5; 1 mM EDTA) to ensure that the DNA concentration does not exceed 100 ng/μl. Too high a concentration of ligation product or too large a volume of ligation product will reduce conversion efficiency and increase the risk of arcing.
7. Be gentle when mixing plasmids. Do not use a pipette tip that is too small when absorbing compatible cells (ordinary 200ul pipette tips should be cut off by 0.6cm). Avoid excessive force, so as not to damage the cell membrane due to excessive shearing force and reduce the Conversion efficiency. Transforming high concentrations of plasmids or ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating.
8. The electroshock competitive cells should be stored below -80°C as much as possible. Over-storage above -80°C will cause the transformation efficiency to decrease.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
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For Research Use Only | Not For Clinical Use.
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