DH5α chemically competent cells (MOFY-0822-FY458)
Cat: MOFY-0822-FY458
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | DH5α strains are commonly used competitive cells in laboratories. The deletion of endonuclease (endA) improves the yield and quality of plasmid DNA; the recombinase-deficient type (recA) reduces the probability of homologous recombination of the inserted fragment and ensures the stability of the inserted DNA; the presence of lacZΔM15 makes DH5α available for blue, Vitiligo screening. |
| Gene Types | F- φ80 lac ZΔM15 Δ (lacZYA-arg F) U169 endA1 recA1 hsdR17 (rk-,mk+) supE44λ- thi -1 gyrA96 relA1 phoA |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 5×108 cfu/μg DNA |
| Note | 1. Competent cells should be slowly thawed in ice, and the target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids or ligation products. 3. Transforming high concentrations of plasmids or high-efficiency ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
Related Products
| MOFY-0822-FY459 | DH5α electro competent cells (MOFY-0822-FY459) |
| MOFY-0822-FY482 | DH5α-λpir chemically competent cells (MOFY-0822-FY482) |
For Research Use Only | Not For Clinical Use.
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