Stbl2 electro competent cells (MOFY-0822-FY471)


Cat: MOFY-0822-FY471
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Specifications

DescriptionThe Stbl2 strain is derived from the JM109 E. coli strain and is suitable for cloning unstable inserts (forward repeats, retroviral sequences, etc.); mcrA mutation and mcrBC-hsd RMS-mrr deletion make this strain more suitable for cloning methylated Genome sequence; meanwhile, Stbl2 can also be used for lentiviral vector construction. Mutation of recA1 and endA1 is beneficial for the stability of cloned DNA and the extraction of high-purity plasmid DNA.
Gene TypesF- mcrA Δ (mcrBC-hsdRMS-mrr) recA1 endA1 lon gyrA96 thi supE44 relA1 λ-Δ (lac-proAB)
Size50 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×1010 cfu/μg DNA
Note1. When adding DNA, the volume should not be greater than 1/10 of the competent volume.
2. When adding electric shock competent cells to the electric shock cup, avoid generating air bubbles, which will increase the risk of arc discharge.
3. When the DNA is impure or contaminated with salt, ethanol, protein and buffer, the transformation efficiency drops sharply.
4. The ions in the shock cup can increase the conductance of the solution, increasing the risk of electric current and arcing in solutions containing cells and DNA.
5. If you want to transform large plasmids or want to obtain higher transformation efficiency, it is recommended to use a high-purity plasmid extraction kit to extract the plasmids. Double the size of the plasmid, and the transformation efficiency drops by an order of magnitude.
6. For the transformation of ligation products, the ligation system or recombination system of some companies (for example: Thermo, NEB's T4 ligase system, NEB, Tiangen's 50-degree reaction recombination system) can be directly mixed with EPI300 electric shock competent Transformation without purification, but the DNA concentration should not be too high, try not to exceed 100 ng/μl. Too high a concentration of ligation product or too large a volume of ligation product will reduce conversion efficiency and increase the risk of arcing.
7. Gently operate when mixing plasmids, avoid excessive force when sucking competitive cells, so as not to damage the cell membrane due to excessive shearing force and reduce the transformation efficiency. Transforming high concentrations of plasmids or ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating.
8. Electroporation of competitive cells should be kept below -80°C as much as possible. Over-storage at temperatures above -80°C will cause the transformation efficiency to decrease.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
Related Products
MOFY-0822-FY472Stbl2 chemically competent cells (MOFY-0822-FY472)
For Research Use Only | Not For Clinical Use.
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