Stbl2 chemically competent cells (MOFY-0822-FY472)


Cat: MOFY-0822-FY472
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Specifications

DescriptionThe Stbl2 strain is derived from the JM109 E. coli strain and is suitable for cloning unstable inserts (forward repeats, retroviral sequences, etc.); mcrA mutation and mcrBC-hsd RMS-mrr deletion make this strain more suitable for cloning methylated Genome sequence; meanwhile, Stbl2 can also be used for lentiviral vector construction.
Gene TypesF- mcrAΔ (mcrBC-hsdRMS-mrr)recA1 endA1 lon gyrA96 thi supE44 relA1λ-Δ (lac-proAB)
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection>5×109 cfu/μg DNA
Note1. Competent cells should be slowly thawed in ice, and the target DNA should be added within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Be gentle when mixing plasmids or ligation products.
3. Transforming high concentrations of plasmids or high-efficiency ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating.
4. Either S.O.C. or LB medium can be used. S.O.C. can improve the transformation efficiency by 20%; experimenters can choose to culture cells at 37°C or 30°C. Under the condition of 37°C, the growth rate of bacteria is accelerated, which is beneficial to increase the yield of plasmids. 30°C Cultivation reduces the probability of false recombination.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
Related Products
MOFY-0822-FY471Stbl2 electro competent cells (MOFY-0822-FY471)
For Research Use Only | Not For Clinical Use.
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