Tuner(DE3)pLysS competent cells (MOFY-0822-FY506)


Cat: MOFY-0822-FY506
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Specifications

DescriptionThe Tuner (DE3) strain is a mutant of the lacZY gene (galactosidyl permease gene) of the BL21 strain. This mutation causes IPTG to enter each cell of E. coli in the system at a uniform rate, resulting in a more stringent and uniform concentration dependence.
Gene TypesF- ompT hsdSB (rB- mB- ) gal dcm lacY1 (DE3)pLysS CamR
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×107 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating.
3. During induction, IPTG concentration can be selected (0.1-10 mM can be used).
4. In order to obtain the required amount of protein, the induction time, temperature and IPTG concentration need to be optimized by the experimenter.
5. The Tuner(DE3) pLysS strain carries the pLysS plasmid. Except for the recovery medium without antibiotics, the other medium and culture medium should contain 34 µg/ml chloramphenicol to prevent the loss of the plasmid.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
Related Products
MOFY-0822-FY507Tuner(DE3) competent cells (MOFY-0822-FY507)
For Research Use Only | Not For Clinical Use.
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