XL1-Blue electro competent cells (MOFY-0822-FY464)


Cat: MOFY-0822-FY464
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DescriptionXL1-Blue electroporation of competitive cells can only be used for electroshock transfer, not for heat shock transfer. The XL1-Blue strain can ensure stable replication of high-copy plasmids, and the mutation of recA1 and endA1 is beneficial to the stability of inserted DNA and the extraction of high-purity plasmid DNA.
Gene TypesrecA1 endA1 gyrA96 thi-1 hsdR17 supE44 (rk-,mk+), relA1 lac [F' proAB lacIqZ∆M15: Tn10 (TetR)]
Size50 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×1010 cfu/μg DNA
Note1. When adding DNA, the volume should not be greater than 1/10 of the competent volume.
2. When adding competitive cells into the electric shock cup, avoid generating air bubbles, which will increase the risk of arc discharge.
3. When the DNA is impure or contaminated with salt, ethanol, protein and buffer, the transformation efficiency drops sharply.
4. The ions in the shock cup can increase the conductance of the solution, increasing the risk of electric current and arcing in solutions containing cells and DNA.
5. If you want to transform large plasmids or want to obtain higher transformation efficiency, it is recommended to use a high-purity plasmid extraction kit to extract the plasmids. Double the size of the plasmid, and the transformation efficiency drops by an order of magnitude.
6. For ligation product transformation, try to ethanol-precipitate the DNA before transformation and resuspend the product with an appropriate amount of TE buffer (10 mM Tris HCl, pH7.5; 1 mM EDTA) to ensure that the DNA concentration does not exceed 100 ng/μl. Too high a concentration of ligation product or too large a volume of ligation product will reduce conversion efficiency and increase the risk of arcing.
7. Be gentle when mixing plasmids. Do not use a pipette tip that is too small when absorbing compatible cells (ordinary 200ul pipette tips should be cut off by 0.6cm). Avoid excessive force, so as not to damage the cell membrane due to excessive shearing force and reduce the Conversion efficiency. Transforming high concentrations of plasmids or ligation products can correspondingly reduce the amount of bacteria that are ultimately used for plating.
8. Electroporation of competitive cells should be kept below -80°C as much as possible. Over-storage at temperatures above -80°C will cause the transformation efficiency to decrease.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
Related Products
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For Research Use Only | Not For Clinical Use.
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