Mouse Anti-cd4 Antibody (CBMOAB-69695FYA)


Cat: CBMOAB-69695FYA
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Lot Number

  • Product List
  • Specifications
  • Application Information
  • Target
  • Reference
  • Relate Reference Data
Sub Cat Clonality Species Reactivity Application Clone Conjugate Size  
CBMOAB-69695FYA Monoclonal Zebrafish (Danio rerio), Cat (Felis catus), Cattle (Bos taurus), Chicken (Gallus gallus), Chicken, Turkey, Quail, Pigeon, Chimpanzee (Pan troglodytes), Dog (Canis lupus familiaris), Feline, Lion, Goat (Capra hircus), Sheep (Ovis aries), Horse (Equus caballus), Human (Homo sapiens), Fruit fly (Drosophila melanogaster), Primate, Rhesus (Macaca mulatta), Mallard (Anas platyrhynchos), Medaka (Oryzias latipes), Mouse (Mus musculus), Pig (Sus scrofa), Porcine, Rabbit (Oryctolagus cuniculus), Rhesus, Human, Mouse WB, ELISA MO69695FYA 100 µg
CBMOAB-00057FYA Monoclonal Cattle (Bos taurus) FC F00057FYA 100 µg
CBMOAB-00058FYA Monoclonal Cattle (Bos taurus) FC F00058FYA 100 µg
CBMOAB-00059FYA Monoclonal Cattle (Bos taurus) FC F00059FYA 100 µg
CBMOAB-00060FYA Monoclonal Cattle (Bos taurus) FC F00060FYA 100 µg
CBMOAB-00138FYA Monoclonal Dog (Canis lupus familiaris) FC F00138FYA 100 µg
CBMOAB-00142FYA Monoclonal Goat (Capra hircus), Sheep (Ovis aries) FC F00142FYA 100 µg
CBMOAB-00149FYA Monoclonal Horse (Equus caballus) FC F00149FYA 100 µg
CBMOAB-00150FYA Monoclonal Horse (Equus caballus) FC F00150FYA 100 µg
CBMOAB-00156FYA Monoclonal Cat (Felis catus) FC F00156FYA 100 µg
CBMOAB-00181FYA Monoclonal Rabbit (Oryctolagus cuniculus) FC F00181FYA 100 µg
MO-NAB-00173W Monoclonal Human (Homo sapiens), Primate, Rhesus (Macaca mulatta) WB, FC, IHC, IHC-P, IP, CyTOF-ready, IF MT310 100 µg
MO-AB-21077W Monoclonal Chimpanzee (Pan troglodytes) WB, ELISA MO21077W 100 µg
MO-AB-29467W Monoclonal Dog (Canis lupus familiaris) WB, ELISA MO29467W 100 µg
MO-AB-36875W Monoclonal Goat (Capra hircus) WB, ELISA MO36875W 100 µg
MO-AB-09834R Monoclonal Cattle (Bos taurus) WB, ELISA MO09834R 100 µg
MO-AB-00183R Monoclonal Medaka (Oryzias latipes) WB, ELISA MO00183R 100 µg
MO-AB-24462R Monoclonal Pig (Sus scrofa) WB, ELISA MO24462R 100 µg
MO-AB-23021H Monoclonal Mallard (Anas platyrhynchos) WB, ELISA MO23021C 100 µg
MO-AB-01062Y Monoclonal Chicken (Gallus gallus) WB, ELISA MO01062Y 100 µg
MO-NAB-00315W Monoclonal Mouse (Mus musculus) ELISA, FC NW0243 100 µg
MO-DKB-00481W Polyclonal Human (Homo sapiens), Fruit fly (Drosophila melanogaster) WB, IHC, IHC-P, IHC-WhMt 100 µg
MOFY-0522-FY2 Monoclonal Chicken, Turkey, Quail, Pigeon FC, IHC, IP, BL 100 µg
MOFY-0522-FY27 Monoclonal Chicken, Turkey FC, IP 100 µg
MOFY-0522-FY52 Monoclonal Porcine FC 100 µg
MOFY-0522-FY71 Monoclonal Feline, Lion FC, IHC 100 µg
MOFY-0722-FY429 Polyclonal Rhesus, Human, Mouse WB, IHC, ICC, IP 100 µg

Specifications

Host speciesMouse (Mus musculus)
Species ReactivityZebrafish (Danio rerio), Cat (Felis catus), Cattle (Bos taurus), Chicken (Gallus gallus), Chicken, Turkey, Quail, Pigeon, Chimpanzee (Pan troglodytes), Dog (Canis lupus familiaris), Feline, Lion, Goat (Capra hircus), Sheep (Ovis aries), Horse (Equus caballus), Human (Homo sapiens), Fruit fly (Drosophila melanogaster), Primate, Rhesus (Macaca mulatta), Mallard (Anas platyrhynchos), Medaka (Oryzias latipes), Mouse (Mus musculus), Pig (Sus scrofa), Porcine, Rabbit (Oryctolagus cuniculus), Rhesus, Human, Mouse
CloneMO69695FYA
SpecificityThis antibody binds to Zebrafish cd4.
FormatLiquid or Lyophilized
StorageStore at 4°C: short-term (1-2weeks)
Store at -20°C: long-term and future use
Purity> 90% was determined by SDS-PAGE
PurificationPurified with Protein A or G affinity chromatography
Cellular LocalizationOther locations; Plasma Membrane

Application Information

ApplicationWB, ELISA
Application NotesELISA: 1:1000-1:3000
Other applications are to be developed. The optimal dilution should be determined by the end user.

Target

IntroductionCD4 (CD4 Molecule) is a Protein Coding gene. Diseases associated with CD4 include Okt4 Epitope Deficiency and Pilonidal Sinus. Among its related pathways are G-protein signaling N-RAS regulation pathway and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and enzyme binding.
Product OverviewMouse Anti-Zebrafish cd4 Antibody is a mouse antibody against cd4. It can be used for cd4 detection in Western Blot, Enzyme-Linked Immunosorbent Assay.
Alternative NamesCD4 protein; cd
UniProt IDU6C9E1
Protein RefseqThe length of the protein is 476 amino acids long.
The sequence is show below: MLGLILIPLFITVLKAQESHEVIYAQVGGTVTIPRDKIERKDGNIKTQDIYVNWFLDSESTPTINRNPQSGSSKVKNARVSLSADFSLQISPVEESDFVIWRCEQHVLAGNYKKTYKLYKVSIPKVPALLVGGHLFLKYVKDVSSVNPSVTWISPKNEGCQENKTTKDTVLVTSVSTCHNGVWTCQLKYGNKKTEATTTVSVIDLAPSPADPIYTSISQSSTVSIPCALSSAIPWSVLNETLQGGSWSFTPLSEPRSPLSLLTLNVGSVVRWDLANGANFTDGKRVITNHNLSIQNLPVKETIRGVYKCSLKFNTKTISREVKVEVLKVSPSGSLKVFEGTRLNVTCSLGHMNTAGLEVKWTCASNCPPFNHKSPPHLSVLSFPNIRMQDKGLVKCELWKNSQKLTSAQLYLRVEKAPVDIWLCVAIGSGVVVFILLVAFAIIYIRRHKQMMMYRRRKTRFCCCNNNKQPKGFYKT.

Reference

Reference1. Yoon, S., Mitra, S., Wyse, C., Alnabulsi, A., Zou, J., Weerdenburg, E. M., ... & Bird, S. (2015). First demonstration of antigen induced cytokine expression by CD4-1+ lymphocytes in a poikilotherm: Studies in zebrafish (Danio rerio). PloS one, 10(6), e0126378.
2. Dee, C. T., Nagaraju, R. T., Athanasiadis, E. I., Gray, C., Fernandez del Ama, L., Johnston, S. A., ... & Hurlstone, A. F. (2016). CD4-transgenic zebrafish reveal tissue-resident Th2-and regulatory T cell-like populations and diverse mononuclear phagocytes. The Journal of Immunology, 197(9), 3520-3530.
3. Miyazawa, R., Matsuura, Y., Shibasaki, Y., Imamura, S., & Nakanishi, T. (2018). Cross-reactivity of monoclonal antibodies against CD4-1 and CD8α of ginbuna crucian carp with lymphocytes of zebrafish and other cyprinid species. Developmental & Comparative Immunology, 80, 15-23.

Relate Reference Data

Figure 1 Zebrafish CD4-1/CD4-2 gene distribution. Cell profile obtained from FACS sorted zf kidney and spleen cells. E = erythrocytes, L = lymphocytes, P = (hemopoietic) precursor cells and M/G = monocytes/granulocytes.

Figure 2 Zebrafish CD4-1 protein expression in transfected CHO cells and zebrafish tissues. Western blot analysis of zfCD4-1 protein.

For Research Use Only | Not For Clinical Use.
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