Rosetta 2(DE3) competent cells (MOFY-0822-FY496)


Cat: MOFY-0822-FY496
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  • Specifications
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Specifications

DescriptionThe pRARE2 plasmid can supplement the tRNA corresponding to 7 rare codons (AUA, AGG, AGA, CUA, CCC, GGA and CGG) lacking in E. coli, and improve the expression level of foreign genes.
Gene TypesF- ompT hsdSB (rB- mB-) gal dcm (DE3) pRARE2 (CamR)
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×108 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating.
3. During induction, IPTG concentration can be selected (0.1-10 mM can be used).
4. In order to obtain the required amount of protein, the induction time, temperature and IPTG concentration need to be optimized by the experimenter.
5. The Rosetta 2(DE3) strain carries the pRARE2 plasmid. Except for the recovery medium without antibiotics, the other medium and culture medium should contain 34 µg/ml chloramphenicol to prevent the loss of the plasmid.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
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For Research Use Only | Not For Clinical Use.
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