Rosetta-gami 2(DE3) competent cells (MOFY-0822-FY497)
Cat: MOFY-0822-FY497
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- Specifications
- Properties
Specifications
| Description | Combines the benefits of both Rosetta 2 and Origami 2 strains. |
| Gene Types | D (ara-leu)7697 DlacX74 DphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F'[lac+ lacIq pro] gor522::Tn10 trxB pRARE2 (CamR, StrR, TetR) |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 1×108 cfu/μg DNA |
| Note | 1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids. 3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. 4. During induction, IPTG concentration is optional (0.1-2 mM can be used). 5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
Related Products
| MOFY-0822-FY496 | Rosetta 2(DE3) competent cells (MOFY-0822-FY496) |
| MOFY-0822-FY498 | Rosetta-gami B(DE3) competent cells (MOFY-0822-FY498) |
| MOFY-0822-FY499 | Rosetta-gami(DE3)pLysS competent cells (MOFY-0822-FY499) |
| MOFY-0822-FY500 | Rosetta(DE3) competent cells (MOFY-0822-FY500) |
For Research Use Only | Not For Clinical Use.
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