Rosetta(DE3) competent cells (MOFY-0822-FY500)


Cat: MOFY-0822-FY500
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  • Specifications
  • Properties

Specifications

DescriptionRosetta (DE3) strain is resistant to chloramphenicol, supplements the tRNA corresponding to 6 rare codons (AUA, AGG, AGA, CUA, CCC, GGA) lacking in E. coli, and improves foreign genes, especially eukaryotic genes, in the Expression levels in prokaryotic systems.
Gene TypesF-ompT hsdSB (rB-mB-) gal dcm (DE3) pRARE (argU, argW, ilex, glyT, leuW, proL) (CamR)
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×108 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Be gentle when mixing plasmids.
3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating.
4. During induction, IPTG concentration is optional (0.1-2mM can be used).
5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
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For Research Use Only | Not For Clinical Use.
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