Mouse Anti-Pig CD8A Antibody (MO-AB-24500R)
Cat: MO-AB-24500R
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- Relate Reference Data
Specifications
Host species | Mouse (Mus musculus) |
Species Reactivity | Pig (Sus scrofa) |
Clone | MO24500R |
Specificity | This antibody binds to Pig CD8A. |
Format | Liquid or Lyophilized |
Storage | Store at 4°C: short-term (1-2weeks) Store at -20°C: long-term and future use |
Purity | > 90% was determined by SDS-PAGE |
Purification | Purified with Protein A or G affinity chromatography |
Application Information
Application | WB, ELISA |
Application Notes | ELISA: 1:1000-1:3000 Other applications are to be developed. The optimal dilution should be determined by the end user. |
Target
Introduction | CD8A (CD8a Molecule) is a Protein Coding gene. Diseases associated with CD8A include Cd8 Deficiency, Familial and Primary Cutaneous Aggressive Epidermotropic Cd8+ T-Cell Lymphoma. Among its related pathways are Hematopoietic Stem Cell Differentiation Pathways and Lineage-specific Markers and T-Cell Receptor and Co-stimulatory Signaling. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and coreceptor activity. |
Product Overview | This product is a mouse antibody against CD8A. It can be used for CD8A detection in Western Blot, Enzyme-Linked Immunosorbent Assay. |
Alternative Names | CD8 antigen alpha protein; CD8A |
UniProt ID | Q6R2U9 |
Protein Refseq | The length of the protein is 236 amino acids long. The sequence is show below: MASLVTALLLPLVLQLHPAKVLGSSLFRTSPEMVQASLGETVKLRCEVMHSNTLTSCSWLYQKPGAASKPIFLMYLSKTRNKTAEGLDTRYISGYKANDNFYLILHRFREEDQGYYFCSFLSNSVLYFSNFMSVFLPAKPTKTPTTPPPKRTPTKASHAVSVAPEVCRPSGNADPRKLDLACDLYNWAPLVGTSGILLLSLVITIICHRRNRRRVCKCPRPVVRQGGKASPSERFI. |
Reference
Reference | 1. Wang, W., Liu, Y., Fu, W., Xu, J., Liu, J., Ding, X., & Zhang, Q. (2015). Single-nucleotide polymorphisms in CD8A and their associations with T lymphocyte subpopulations in pig. Molecular Genetics and Genomics, 290, 1447-1456. 2. Dawson, H. D., Royaee, A. R., Nishi, S., Kuhar, D., Schnitzlein, W. M., Zuckermann, F., ... & Lunney, J. K. (2004). Identification of key immune mediators regulating T helper 1 responses in swine. Veterinary immunology and immunopathology, 100(1-2), 105-111. |
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Figure 1 T and B sub-population proliferation monitored by CFSE labelling in response to in vitro stimulation with PHA. PBMC proliferation in a pig whose CFSE-labelled cells were cultured in vitro in the presence of PWM or in media only (CTR). After 5 days, cells were stained with anti-CD8a and anti-CD79a monoclonal antibody and analysed by flow cytometry.
Reference: Vitali, L. A., Beghelli, D., Nya, P. C. B., Bistoni, O., Cappellacci, L., Damiano, S., ... & Bramucci, M. (2016). Diverse biological effects of the essential oil from Iranian Trachyspermum ammi. Arabian Journal of Chemistry, 9(6), 775-786.
For Research Use Only | Not For Clinical Use.
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