BL21(DE3) competent cells (MOFY-0822-FY494)


Cat: MOFY-0822-FY494
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Specifications

DescriptionHigh-level expression of non-toxic proteins, which can be used for protein expression of pET series, pGEX, pMAL and other plasmids at the same time;
The efficiency of electrotransformation can reach 1×1010 cfu/μg DNA, which can be used for the construction of various polypeptide and protein expression libraries
Gene TypesF- ompT hsdSB (rB- mB- ) gal dcm (DE3)
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×107 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Be gentle when mixing plasmids.
3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating.
4. During induction, IPTG concentration is optional (0.1-2mM can be used).
5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
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For Research Use Only | Not For Clinical Use.
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