BL21(DE3) competent cells (MOFY-0822-FY494)
Cat: MOFY-0822-FY494
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
| Size: | |
| Inquiry |
- Product Details
Specifications
| Description | High-level expression of non-toxic proteins, which can be used for protein expression of pET series, pGEX, pMAL and other plasmids at the same time; The efficiency of electrotransformation can reach 1×1010 cfu/μg DNA, which can be used for the construction of various polypeptide and protein expression libraries |
| Gene Types | F- ompT hsdSB (rB- mB- ) gal dcm (DE3) |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 1×107 cfu/μg DNA |
| Note | 1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids. 3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. 4. During induction, IPTG concentration is optional (0.1-2mM can be used). 5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
Related Products
| MOFY-0822-FY488 | BL21(DE3) electro competent cells (MOFY-0822-FY488) |
| MOFY-0822-FY489 | BL21-CodonPlus (DE3)-RIPL competent cells (MOFY-0822-FY489) |
| MOFY-0822-FY490 | BL21 Star(DE3)pLysS competent cells (MOFY-0822-FY490) |
| MOFY-0822-FY491 | BL21 Star(DE3) competent cells (MOFY-0822-FY491) |
| MOFY-0822-FY492 | BL21(AI) competent cells (MOFY-0822-FY492) |
| MOFY-0822-FY493 | BL21(DE3)pLysS competent cells (MOFY-0822-FY493) |
| MOFY-0822-FY495 | BL21 competent cells (MOFY-0822-FY495) |
For Research Use Only | Not For Clinical Use.
Online Inquiry