BL21 Star(DE3) competent cells (MOFY-0822-FY491)
Cat: MOFY-0822-FY491
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To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Specifications
| Description | The BL21 Star (DE3) strain is derived from the BL21 (DE3) strain and contains the rne131 mutation (RNaseE gene). The mutation of the RNaseE gene reduces the accumulation of endogenous RNase and enhances the stability of mRNA in the strain cells, thereby improving the expression of heterologous proteins. Level. |
| Gene Types | F- ompT hsdSB (rB- mB- ) gal dcm rne131 (DE3) |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 1×108 cfu/μg DNA |
| Note | 1. The competent cells should be thawed slowly in ice as much as possible, and the target DNA should be added within 8 minutes of inserting into the ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids. 3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. 4. During induction, IPTG concentration is optional (0.1-2mM can be used). 5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
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For Research Use Only | Not For Clinical Use.
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