BL21 Star(DE3)pLysS competent cells (MOFY-0822-FY490)
Cat: MOFY-0822-FY490
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Specifications
| Description | BL21 Star(DE3)pLysScompetent cells The basal expression level of heterologous protein is improved, and it can be used for protein expression of pET series, pGEX, pMAL and other plasmids at the same time |
| Gene Types | F- ompT hsdSB (rB- mB- ) gal dcm rne131 (DE3)pLysS (CamR) |
| Size | 100 µL/vial |
| QC result | Transformation efficiency of pUC19 plasmid detection:> 1×107 cfu/μg DNA |
| Note | 1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency. 2. Be gentle when mixing plasmids. 3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating. 4. During induction, IPTG concentration is optional (0.1-2mM can be used). 5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter. 6. The BL21 Star(DE3)pLysS strain carries the pLysS plasmid. Except for the recovery medium without antibiotics, the other medium and medium should contain 34 µg/ml chloramphenicol to prevent the loss of the plasmid. |
Properties
| Shipping | Dry ice |
| Storage | Store at -80 °C |
| Handling Advice | Immediately upon receipt, cells were transferred from dry ice to -80°C refrigerator. |
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For Research Use Only | Not For Clinical Use.
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