BL21 competent cells (MOFY-0822-FY495)


Cat: MOFY-0822-FY495
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Size:
 Inquiry
  • Specifications
  • Properties

Specifications

DescriptionHigh-level expression of non-toxic proteins, cannot be used for protein expression driven by T7 promoter (such as pET series)
Gene TypesE. coli B F- dcm ompT hsdS (rB- mB-) gal [malB+]K-12 (λS)
Size100 µL/vial
QC resultTransformation efficiency of pUC19 plasmid detection:> 1×107 cfu/μg DNA
Note1. Melt competent cells slowly in ice as much as possible, and add target DNA within 8 minutes of inserting into ice. Do not keep in ice for too long, as long-term storage will reduce the transformation efficiency.
2. Be gentle when mixing plasmids.
3. Transforming high concentrations of plasmids can correspondingly reduce the amount of bacteria that are ultimately used for plating.
4. During induction, IPTG concentration is optional (0.1-2 mM can be used).
5. In order to obtain the required amount of protein, the induction time, temperature, and IPTG concentration need to be optimized by the experimenter.

Properties

ShippingDry ice
StorageStore at -80 °C
Handling AdviceImmediately upon receipt, cells were transferred from dry ice to -80°C refrigerator.
Related Products
MOFY-0822-FY488BL21(DE3) electro competent cells (MOFY-0822-FY488)
MOFY-0822-FY489BL21-CodonPlus (DE3)-RIPL competent cells (MOFY-0822-FY489)
MOFY-0822-FY490BL21 Star(DE3)pLysS competent cells (MOFY-0822-FY490)
MOFY-0822-FY491BL21 Star(DE3) competent cells (MOFY-0822-FY491)
MOFY-0822-FY492BL21(AI) competent cells (MOFY-0822-FY492)
MOFY-0822-FY493BL21(DE3)pLysS competent cells (MOFY-0822-FY493)
MOFY-0822-FY494BL21(DE3) competent cells (MOFY-0822-FY494)
For Research Use Only | Not For Clinical Use.
Online Inquiry