Bestrophin-1
Bestrophin calcium-activated chloride channels (CaCCs) regulate the flow of chloride and other monovalent anions across cellular membranes in response to intracellular calcium (Ca(2+)) levels. Mutations in bestrophin 1 (BEST1) cause certain eye diseases. Here we present X-ray structures of chicken BEST1-Fab complexes, at 2.85 Å resolution, with permeant anions and Ca(2+). Representing, to our knowledge, the first structure of a CaCC, the eukaryotic BEST1 channel, which recapitulates CaCC function in liposomes, is formed from a pentameric assembly of subunits. Ca(2+) binds to the channel's large cytosolic region. A single ion pore, approximately 95 Å in length, is located along the central axis and contains at least 15 binding sites for anions. A hydrophobic neck within the pore probably forms the gate. Phenylalanine residues within it may coordinate permeating anions via anion-π interactions. Conformational changes observed near the 'Ca(2+) clasp' hint at the mechanism of Ca(2+)-dependent gating. Disease-causing mutations are prevalent within the gating apparatus.
Mouse Anti-D. melanogaster Best1 Antibody (CBMOAB-02375FYA) (CAT#: CBMOAB-02375FYA)
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- Host species: Mouse
- Species Reactivity: Fruit fly (Drosophila melanogaster)
- Application: WB, ELISA
- Protein: Bestrophin homolog
- Size: 0.5mg, 1mg, 200µg
- Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
- Alternative Names: Bestrophin 1, Bestrophin 1, isoform A, Dbest, LP05915p, Best1
Mouse Anti-C. elegans BEST1 Antibody (CBMOAB-00900HCB) (CAT#: CBMOAB-00900HCB)
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- Host species: Mouse
- Species Reactivity: C. elegans (Caenorhabditis elegans)
- Application: WB, ELISA
- Protein: Bestrophin homolog 1
- Size: 0.5mg, 1mg, 200µg
- Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
- Alternative Names: Bestrophin homolog 1, best-1
Mouse Anti-Zebrafish best1 Antibody (CBMOAB-67627FYA) (CAT#: CBMOAB-67627FYA)
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- Host species: Mouse
- Species Reactivity: Zebrafish (Danio rerio)
- Application: WB, ELISA
- Size: 0.5mg, 1mg, 200µg
- Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
- Alternative Names: best1, CU651670.1, Bestrophin 1
Mouse Anti-Rhesus BEST1 Antibody (MO-AB-01288W) (CAT#: MO-AB-01288W)
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- Host species: Mouse
- Species Reactivity: Rhesus (Macaca mulatta)
- Application: WB, ELISA
- Protein: Bestrophin
- Size: 0.5mg, 1mg, 200µg
- Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
- Alternative Names: Bestrophin-1 isoform 1, BEST1
Mouse Anti-Cattle BEST1 Antibody (MO-AB-08058R) (CAT#: MO-AB-08058R)
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- Host species: Mouse
- Species Reactivity: Cattle (Bos taurus)
- Application: WB, ELISA
- Protein: Bestrophin
- Size: 0.5mg, 1mg, 200µg
- Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
- Alternative Names: Bestrophin 1, BEST1
Mouse Anti-Pig BEST1 Antibody (MO-AB-24121R) (CAT#: MO-AB-24121R)
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- Host species: Mouse
- Species Reactivity: Pig (Sus scrofa)
- Application: WB, ELISA
- Protein: Bestrophin
- Size: 0.5mg, 1mg, 200µg
- Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
- Alternative Names: Bestrophin-1, BEST1
Mouse Anti-Dog BEST1 Antibody (MO-AB-29237W) (CAT#: MO-AB-29237W)
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- Host species: Mouse
- Species Reactivity: Dog (Canis lupus familiaris)
- Application: WB, ELISA
- Protein: Bestrophin
- Size: 0.5mg, 1mg, 200µg
- Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
- Alternative Names: Bestrophin, VMD2, BEST1
Mouse Anti-Marmoset BEST1 Antibody (MO-AB-51847W) (CAT#: MO-AB-51847W)
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- Host species: Mouse
- Species Reactivity: Marmoset
- Application: WB, ELISA
- Protein: Bestrophin
- Size: 0.5mg, 1mg, 200µg
- Conjugate: Alexa Fluor 350, AP, APC, Biotin, Consult us more, Cy3, Cy5, Cy5.5, Cy7, FITC, HRP, NONE, PE, PerCP
- Alternative Names: Bestrophin-1 isoform 1, BEST1
Anti-BEST1 Antibody (Cat MORAB-027F), Mouse IgG (CAT#: MORAB-027F)
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- Host species: Mouse
- Species Reactivity: Chicken (Gallus gallus)
- Application: IA
- Protein: Submitted name: Uncharacterized protein
- Size: 0.5mg, 1mg, 10mg
- Conjugate: None
- Alternative Names: Best1 bestrophin-1, BEST1
