Overview
Creative Biolabs Zebrafish gene editing technology specializes in custom zebrafish gene editing strain construction. Professional gene editing technology services include zebrafish gene knock-in, knock-out, silencing, point mutation, transgenic services, and the development of zebrafish disease models. The zebrafish technology service platform employs highly skilled technicians and analysts capable of providing high-quality zebrafish gene editing services.
Applications of Zebrafish Gene Editing
- Growth Regulation of the Zebrafish nos2a Gene
CRISPR/Cas9 gene editing technology was used to create a nos2a-deleted (nos2a-/-) zebrafish line that can be inherited stably. The findings revealed that the loss of nos2a function in zebrafish inhibited fish growth, with down-regulation of gh1 and igf1 mRNA levels being one possible explanation.
- Establishment of Zebrafish Morn3 Gene Knockout Model Using CRISPR/Cas9 System
- Creating a Zebrafish Morn3 Gene Knockout Model Using the CRISPR/Cas9 System
CRISPR/Cas9 gene editing system successfully edited the Morn3 gene of zebrafish, yielding Morn3 gene-edited mutants.
- Screening of Zebrafish hoxb4 Gene and its Mutant Genotypes Edited by CRISPR/Cas9 System
The CRISPR/Cas9 system successfully edited the zebrafish hoxb4 gene, and its mutation type was identified by sequencing, providing a reliable gene knockout method for studying the function of the HOXb4 gene.
Creative Biolabs offers a zebrafish gene editing platform that meets the highest quality and efficiency standards. Because of the largest scale and highest standard single zebrafish breeding system, as well as a high-quality professional talent team. Gene editing technology will have significant research and application value in basic biology, medicine, biotechnology, and other fields in the future.
CRO Service
The most important technical tool in reverse genetics research is gene editing technology. Zebrafish with gene knockouts are widely used in genetics, developmental biology, cell biology, medicine, environmental toxicology, aquatic breeding, and other fields of study. Mutagenesis technology, Tol2 transposon, Morpholino, TALEN, and CRISPR/Cas system are examples of zebrafish gene editing technology methods.
Zebrafish Transgenic Techniques
Microinjection, electroporation, sperm carrier method, GAL4/UAS transcriptional activation system, Tol2 transposon-mediated, mimic retrovirus-mediated, recombinant baculovirus mediation, and other methods are used to create transgenic zebrafish.
- Transgenic zebrafish for studies of gene expression and developmental regulation.
- In vivo morphogenesis and cell migration studies.
- Research on human immune diseases, autophagy, and neurological diseases.
- Fluorescent fish to screen for mutants, small molecules that block specific developmental pathways, or toxins that inhibit normal development.
- Transgenic zebrafish for ecological environment monitoring.
Zebrafish Gene Knock In
Non-homologous DNA repair technology inserts exogenous DNA fragments (fluorescent proteins, PA, and so on) into the zebrafish genome.
- Unique and efficient zebrafish screening method.
Zebrafish Gene Knock Out
The target is designed and the target gRNA is prepared in the conserved domain or the customer-specified region, and the gRNA is co-injected to cause a frameshift mutation in the gene, and then a stable genetic strain is obtained by PCR screening, according to the zebrafish gene.
- Mature gene knockout technology quickly obtains stable zebrafish strains.
- Multi-target co-injection of zebrafish F0 generation to verify gene function.
Creative Biolabs provides modelorg technical platform and antibody for zebrafish, as well as custom service to meet your specific needs. Please do not hesitate to contact us.